辽东楤木对一次力竭小鼠血淋巴细胞DNA损伤的影响

Effect of Aralia Elata on DNA Damage of Lymphocyte in Blood of Mouses After Exhaustive Exercise

目的:急性运动可引起氧自由基产生增加,从而导致机体一系列病理损伤。通过给小鼠补充辽东楤木根皮提取物,5周后进行急性一次力竭运动,观察运动源性自由基对血淋巴细胞DNA的损伤情况以及血清丙二醛(MDA)含量、血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性的变化,探讨辽东楤木对一次性力竭性运动后机体自由基代谢的影响。方法:昆明种小鼠64只,随机分为四组:正常对照组(NC组)16只、运动对照组(EC组)16只、运动+辽东楤木组(EA组)16只,运动+人参组(EP组)16只、除正常对照组外,其他各组小鼠于最后一次灌胃后,次日晨进行一次力竭游泳运动后,取样。采用单细胞凝胶电泳法、硫代巴比妥酸法、黄嘌呤氧化酶法和二硫代二硝基苯甲酸法分别测定血液淋巴细胞DNA的损伤、血清MDA的含量、血清SOD和GSH-Px的活性。结果:(1)与NC组相比,EC组大鼠血清MDA含量显著增加(P<0.01),血清SOD和GSH-Px活性显著下降(P<0.01)。与EC组相比,EA组大鼠血清MDA含量显著下降(P<0.01),血清SOD和GSH-Px活性显著增高(P<0.01);EP组大鼠血清MDA含量显著下降(P<0.01),血清SOD和GSH-Px活性显著增高(P<0.01)。(2)与NC组相比,EC组大鼠血液淋巴细胞尾长(Tail length)、尾矩(Tail moment)及椭圆矩(Olive moment)显著增加(P<0.01)。与EC组相比,EA组大鼠血液淋巴细胞Tail length、Tail moment、Olive moment显著降低(P<0.01);EP组大鼠血液淋巴细胞尾长、尾短和椭圆矩显著降低(P<0.01)。结论:1)辽东楤木可以提高小鼠血清SOD、GSH-Px的活性,降低血清MDA的含量,减少OH.等对DNA的直接损伤。2)辽东楤木对一次力竭运动导致的小鼠血液淋巴细胞DNA的损伤具有保护作用,与人参的作用效果相类似。

Objective：Free radical will increase after acute exercise,which will induce some damage in economy.This article was to observe that exhaustive exercise harms the DNA of lymphocyte and whether Aralia elata has a role in protecting the DNA damage of lymphocyte after exhaustive exercise,in order to provide the experimental evidences for Aralia elata staving and eliminating exercise-induced fatigue.Methods：Eighty mouses were divided into 4 groups at random：normal contrast group（NC,n=16）,exercise contrast group（EC,n=16）,exercise and take Aralia elata group（EA,n=16）,exercise and take Panax ginseng group（EP,n=16）.We adopted exhaustive exercise.For making sure there were 8 rats in every group,the authors eliminated the superabundance rats randomly after the exercise.The rats were sacrificed and sampled after exercise.Measured the DNA damage of lymphocyte,malondialdehyde（MDA） content,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） activities in serum of rats by SCGE,TBA,XO and DTNB methods.Results：（1）Compared with NC group,the MDA content increased remarkably（P0.01）,the SOD and GSH-Px activities in serum of rats decreased markedly in EC group（P0.01）.Compared with EC group,the MDA content decreased remarkably（P0.01）,the SOD and GSH-Px activity in serum of rats increased markedly（P0.01） in EA group;the MDA content decreased markedly（P0.01）,the SOD and GSH-Px activities in serum of rats increased remarkably in EP group rats（P0.01）.（2）Compared with NC group,Tail length,Tail moment and Olive moment of lymphocyte increased observably in EC group rats（P0.01）.Compared with EC group,Tail length,Tail moment and Olive moment in EA group rats decreased markedly（P0.01）;there were an obvious decrease in Tail length,Tail moment and Olive moment（P0.01）in EP group rats.Conclusion：（1） Aralia elata can eliminate the free radicals,enhance the SOD and GSH-Px activities in serum of rats and reduce the damage of DNA caused by OH· etc.（2） Aralia elata can attenuate exercise-induced DNA damage after exhaustive exercise.The function is similar with Panaxginseng.