单核细胞趋化蛋白1和8异前列腺素F2α与糖尿病肾病相关性的实验研究

The Experimental Study of the Correlation between Monocyte Chemoattractant Protein-1,8-iso-prostaglandin F2a and Diabetic Nephropathy

目的探讨单核细胞趋化蛋白1(MCP-1)和8异前列腺素F2α(8-iso-PGF2α)与糖尿病肾病(DN)的关系。方法29只雄性Wistar大鼠随机分为正常对照组(n=10)和DN模型组(n=19)。采用高脂高糖饮食和腹腔小剂量注射链脲佐菌素诱导糖尿病模型,成功后持续喂养8周,作为DN模型。检测空腹血糖和24h尿白蛋白排泄率,ELISA法检测血尿MCP-1和血8-iso-PGF2α。结果DN模型组的血糖、24h尿白蛋白排泄率、血尿MCP-1和血8-iso-PGF2α均明显高于对照组(P均<0.01);MCP-1、8-iso-PGF2α均与24h尿白蛋白排泄率呈正相关(r=0.92,P<0.01;r=0.83,P<0.01)。结论炎症和氧化应激反应在DN的发生、发展中可能起重要作用。

Objective To check the correlation between monocyte chemoattractant protein-1（MCP-1）,8-iso-prostaglandin F2α（8-isoPGF2α）and diabetic nephropathy.Methods 29 rats were divided into nomal control group（n=10）,diabetic nephropathy model group（n =19）.The rats were feeded with high lipoids and high glucose food,injected small dose strepozotocin into the abdominal cavity to induce the analog of diabetes.After succeeding,the rats were feeded up 8 weeks and became diabetic nephropathy model.To detect fasting blood-glucose and urine albumin excretion rate（UAER）of 24 hours,and detected MCP-1,8-iso-PGF2α through ELISA method.Results The blood glucose,UAER,MCP-1 and 8-iso-PGF2α of model group were increased obviously than the normal group（P〈0.01）.There were positive correlation between 8-iso-PGF2α,MCP-1 and 24 h UAE.Conclusion Inflamation and oxidative stress reaction possibly played an important role in the occurrence and development of diabetic nephropathy.