早期肝素治疗对重症急性胰腺炎大鼠胰腺组织微血栓形成的影响

Effection of Early Administration of Heparin on Pancreatic Microthrombosis in Rat Model of Severe Acute Pancreatitis

目的观察和评价早期肝素治疗对重症急性胰腺炎(SAP)大鼠胰腺组织微血栓形成的影响和疗效。方法SD大鼠随机分为SAP组、肝素组和假手术组。12h检测血清淀粉酶、血浆D-二聚体(D-dimer)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6);采集胰腺行普通病理检查并计数微血栓。结果SAP诱发后12h,SAP组大鼠的存活率显著低于肝素组和假手术组(P<0.05);胰腺组织病理学观察显示,肝素组微血栓数、病理评分显著少于SAP组(P<0.05);血清淀粉酶、D-dimer、TNF-α以及IL-6检测结果也显示肝素组较SAP组显著改善(P<0.05)。结论早期肝素治疗能减轻胰腺病理损害,减少胰腺组织微血栓数量,降低全身炎性反应,提高SAP大鼠的生存率。

Objective To evaluate early administration of heparin on pancreatic microthrombsis in rats with severe acute pancreatiti（sSAP）.Methods SD rats were allocated into 3 groups randomly：SAP group,heparin group,and sham operation group.12 hours after the operation,all animals were detected the serum amylase,plasma D-dimer,TNF-α and IL-6 levels.The samples of the pancreas were collected for pathologic examination under light microscopy .The numbers of pancreatic microthrombi were also counted with microscopy.Results At 12 hours after modelization,the survival rate in SAP group was significantly lower than heparin and sham operation groups（P〈0.05）.The numbers of microthrombi in the pancreas and the pathological scoring of pancreatic injury in heparin group were significantly fewer than those in SAP group（P〈0.05）.The level of serum amylase,plasma D-dimer,TNF-α and IL-6 also showed significant improvement in heparin group compared with those in SAP group（P〈0.05）.Conclusion Early administration of heparin increases survival rate of SAP rats with less histological injury and microthrombi.It also can improve coagulation parameters including D-dimer and alleviate inflammatory response.