槐耳清膏体外诱导人肝癌细胞MHCC97H凋亡

Trametes Robiniophila Murr Induced Apoptosis of Hepatocellular Carcinoma Cell Line MHCC97H

[目的]研究槐耳清膏对人肝癌细胞MHCC97H凋亡的作用及其机制。[方法]不同浓度槐耳清膏及5-Fu分别与人肝癌细胞MHCC97H共同培养,采用CCK-8法、FCM及荧光显微镜检测细胞增殖与凋亡变化,细胞免疫组化检测Bax、Bcl-2及p53基因的表达。[结果]槐耳清膏在体外可抑制MHCC97H细胞的增殖,诱导MHCC97H细胞产生早期凋亡,且作用具有剂量和时间依赖性。荧光显微镜下细胞呈典型的早期凋亡形态学改变。细胞免疫组化可见Bax及p53蛋白表达增强,而Bcl-2蛋白表达减少。[结论]槐耳清膏能明显诱导人肝癌细胞MHCC97H凋亡,其机制可能与改变凋亡相关基因Bax、Bcl-2、p53的表达有关。

[Purpose] To investigate the effect and mechanism of Trametes robiniophila Murr induced apoptosis of human hepatocellular carcinoma cell line MHCC97H. [Methods] MHCC97H cells were incubated with different dose of Trametes robiniophila Murr or 5-Fu. Cell growth and apoptosis were assessed by CCK-8, flow cytometr （FCM） and fluorescence microscopy. The change of Bax, Bcl-2 and p53 expression were detected by cell immunohistochemistry. [Results] The growth of MHCC97H cells was inhibited and the apoptosis was induced by Trametes robiniophila in vitro in a time/dose-dependent manner. Typical morphological changes of early apoptosis could be observed by fluorescence microscopy. Cell immunohistochemistry showed that the expression of Bax and p53 was upregulated and expression of Bcl-2 was downregulated. [Conclusion] Trametes robiniophila Murr can markedly induce apoptosis in human hepatocellular carcinoma cell line MHCC97H. The mechanism might be related to the change of Bax, Bcl-2 and p53 expression.