VEGF和CD_(34)在骨肉瘤组织中的表达及临床意义

Expression of VEGF and CD34 in osteosarcoma tissue and its clinical significance

目的:探索VEGF和CD34在骨肉瘤组织中的表达,分析VEGF与微血管密度(MVD)及转移预后的关系。方法:应用免疫组织化学法(S-P法)检测41例骨肉瘤组织中VEGF和CD34的表达,计算MVD值,并结合临床病例资料综合分析。结果:41例骨肉瘤患者中,VEGF阳性表达率为68.2%(28/41),与肿瘤大小、Price分级、Dahlin,s组织学分型等无关(P>0.05);所有病例CD34表达均为阳性。VEGF阳性组MVD为(40.25±8.02)in,与阴性组MVD为(15.54±5.87)in相比,差异有显著性(P<0.05)。转移组VEGF的阳性率为92.9%,明显高于无转移组VEGF的阳性率55.6%,差异有显著性(P<0.05)。VEGF阳性组的5年存活率为7.1%,明显低于VEGF阴性组5年存活率46.2%,差异有显著性(P<0.05)。结论:VEGF在骨肉瘤组织中高表达,VEGF的表达与MVD值、转移预后显著相关,检测VEGF的阳性表达和MVD值可以作为判断骨肉瘤转移和预后的重要指标。

Objective：To explore the expression of VEGF and CD34 in osteosarcoma tissue and to analyze the association of VEGF with MVD,metastasis and prognosis of osteosarcoma.Methods：Immunohisto-chemical staining（SP method）was used to detect the expressions of VEGF and CD34 in specimens from 41 cases of osteosarcoma.To calculate microvessel density and overall analyze information of all the 41 cases.Results：In 41 cases,the positive rate of VEGF was 68.2%（28/41）.The expression of VEGF was not significantly correlated to tumor＇s size,Price,s grade and Dahlin＇s difierentiation （P〉0.05）;the expression of CD34 in all the 41 cases was positive. MVD （40.25±8.02）in VEGF positive patients was much higher than that（15.54±5.87）in VEGF negative patients（P〈0.05）;the positive rate of VEGF （92.9%） in the patients with metastasis was remarkably higher than that （55.6%）in the patients without metastasis （P〈0.05）;the five-year survival rate （7.1%）in the patients with positive expression of VEGFwas significantly lower than that （46.2%）in negative control groug （P〈0.05）.Conclusion：High expression of VEGF is found in osteosarcoma tissue.The expression of VEGF is closely correlated to MVD,metastasis and prognosis of osteosarcoma.Detection of VEGF and MVD may be useful for judging metastasis and prognosis of osteosarcoma.