猪繁殖与呼吸综合征病毒BJ株、GD株全基因组序列测定与分析

Genomic Sequencing of BJ Strain and GD Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate

利用RT-PCR方法分别扩增出猪繁殖与呼吸综合征病毒(PRRSV)北京分离株(BJ株)、广东分离株(GD株)各9条基因片段,并将这9个片段分别克隆于PGEM-T-easy质粒载体上进行测序,按顺序将这些序列进行拼接得到PRRSVBJ株和GD株的全基因组cDNA(GenBank:EU825723,EU825724)。测序结果表明,BJ株和GD株基因组全长分别为15340和15336bp,各包含9个开放式阅读框,5′UTR都含有189nt,3′端URT分别含有170nt、166nt,其中各包含20ntPoly(A)、16ntPoly(A)。基因组序列分析结果显示,BJ株和GD株与ATCCVR-2332的核苷酸同源性分别为88.9%和89.0%,与高致病性PRRSV毒株JXA1的核苷酸同源性分别为99.0%和99.4%,与PRRSV毒株HuN的核苷酸同源性分别为98.8%和98.9%。

Nine gene fragments of BJ strain and GD strain of procine reproductive and respiratory syndrome virus were amplified by RT-PCR and cloned into the PGEM-T-easy plasmid vector respectively and sequenced.Complete nucleotide sequence of genome of PRRSV BJ and GD were obtained by spicing sequence of each fragment in order（GenBank：EU825723,EU825724）.It showed that the genome of PRRSV BJ and GD were 15 340 bp and 15 336 bp in length,containing nine open reading frames（ORFs） with 189 nt leader sequence in the 5＇ end of the viral genome and 170 nt,166nt uncoding terminal region（UTR） in the 3＇ end of the genome,including 20nt Poly（A） and 16nt Poly（A） tail respectively.The genome of PRRSV BJ and GD strain showed 88.9%,89.0%;99.0%,99.4%and 98.8%,98.9%of nucleotide indentity with ATCC VR-2332,JXA1 and HuN,respectively.