西藏半野生小麦高分子量麦谷蛋白亚基遗传多样性分析

Genetic Diversity Analysis on High Molecular Weight Glutenin Subunits of Triticum aestivum ssp.tibetanum Shao

利用SDS-PAGE技术对142份西藏半野生小麦的HMW-GS的组成进行了遗传多样性分析。结果表明,在供试材料中,Glu-1位点共有12种等位基因,Glu-A1位点的1（2.82%）和null（97.18%）亚基,Glu-B1位点的8（0.7%）、7＋8（80.28%）、6＋8（16.20%）、7＋9（2.11%）、23＋24（0.7%）亚基和Glu-D1位点的2＋12（91.55%）、2＋10（2.11%）、4＋12（2.11%）、2＋12＊（1.41%）、2（2.82%）亚基;其中亚基null、7＋8、2＋12在各自的位点上出现频率最高,分别达到了97.18%、80.28%、91.55%;亚基组合共有12种,主要为null/7＋8/2＋12,频率高达73.24%。根据获得的HMW-GS谱带构建[1,0]矩阵,计算材料间的Nei-Li′遗传相似系数（GS）,发现所有材料间GS值变化范围在0.40~0.66之间,平均值为0.53。在142份供试材料中,共发现12条相对迁移率不同的谱带,频率变异的范围为0.7%~97.18%,Glu-A1、Glu-B1、Glu-D1三个位点的多样性指数分别为0.128、0.632、0.404,平均值为0.388。

In order to understand the composition and distribution of high molecular weight subunit（HMW-GS）in semi-natural wheat in Tibet,142 semi-natural wheat landraces in Tibet were analyzed by means of SDS-PAGE.The result indicated that there were 12 alleles on Glu-1 locus,the primary alleles were 1（2.82%）and Null（97.18%）on Glu-A1,8（0.7%）,7＋8（80.28%）,6＋8（16.20%）,7＋9（2.11%）,23＋24（0.7%）on Glu-B1 and 2＋12（91.55%）,2＋10（2.11%）,4＋12（2.11%）,2＋12＊（1.41%）,2（2.82%）on Glu-D1;The frequencies of null,7＋8 and 2＋12 alleles were the highest,reaching 97.18%,80.28% and 91.55% respectively.There are 12 subunit combinations and the predominant type was null,7＋8 and 2＋12 with a frequency of 73.24%.Result from cluster analysis showed that genetics similarities among 142 landraces ranged from 0.40 to 0.66 with an average of 0.53.The results indicated that 12 bands of HMW-GS were observed in all of the 142 materials,with the frequencies varied from 0.7% to 97.18%,as well as the alleles diversity indexes on A1,B1 and D1 were 0.128,0.632 and 0.404 respectively,with an average of 0.388.