摘要
HOG1(high osmolarity glycerol,HOG1)是酵母中参与耐高渗透压调控的重要基因。根据已发表的酿酒酵母序列,设计特异引物,扩增到完整的HOG1基因;构建植物表达载体pBI121-HOG1,通过农杆菌介导转化拟南芥,获得转化植株种子。对收获的T0代种子进行卡那霉素抗性筛选,并通过PCR和RT-PCR对抗性苗进行检测。结果表明,构建的载体成功转化拟南芥,并获得了13份转HOG1基因苗。
HOG1 is an important regulatory factor which regulates response to high osmolarity in Yeast.In this paper,the full-length HOG1 gene from the genomic DNA ofSaccharomyces cerevisiae was isolated with PCR technique using primers designed according to the published sequence.To identify potential function of this gene in plant,expression vector pBI121-HOG1 was constructed,and then transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method.T0 transgenic seeds were screened on MS plate containing Kanamycin.After PCR and RT-PCR,13 positive transgenic seedlings were identified,providing material for further studies on the function of HOG1 gene and its interaction with other genes in plants.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第4期86-90,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
教育部科技创新工程重大项目培育基金(707054)
高等学校学科创新引智计划资助(111-2-16)
国家"十一五"
"863"计划-现代节水农业技术系统创新及集成应用(2006AA100223)
关键词
HOG1基因
构建植物表达载体
拟南芥转化
HOG1 gene
Construction of plant expression vector
Arabidopsis thaliana transformation
