HPLC法测定螺内酯片的含量

Content Determination of Spironolactone Tablets by HPLC

目的:建立以高效液相色谱法测定螺内酯片含量的方法。方法:色谱柱为AgilentC18,流动相为乙腈-水(50∶50),测定波长为238nm。结果:螺内酯检测浓度线性范围为5.0231～75.3471μg·mL-1(r=1.0000);平均回收率为100.23%,RSD=0.53%。结论:该方法重复性好,操作简便,可用于螺内酯片的质量控制。

OBJECTIVE： To establish the HPLC method for the determination of spironolactone tablets. METHODS： The determination was performed on Agilent C18 column with mobile phase of acetonitrile-water （50 ∶ 50） and detection wavelength of 238 nm. RESULTS： The linear range of spironolactone were within 5.023 1 μg·mL-1～75.347 1 μg·mL-1 （r=1.000 0） with an average recovery of 100.23% （RSD=0.53%）. CONCLUSION： The method is reproducible, simple and convenient for the quality control of spironolactone tablets.