黄牛Gli3基因第10外显子多态性及其与生长性状相关性

Polymorphism of the Exon 10 of Gli3 Gene and Correlation with Growth Traits in Cattle

利用PCR-SSCP和DNA测序技术研究708头黄牛Gli3基因第10外显子的多态性,发现此外显子存在SSCP多态。对不同SSCP带型的对应片段进行测序分析,共发现2个新的SNP多态位点(NW_001494928:g.205649T>C,205754A>C),这2个SNP完全连锁。南阳牛不同基因型与生长发育性状的相关性分析显示,CC基因型个体的24月龄平均日增体质量指标显著大于TT基因型个体(P<0.05),其余各项指标基因型间差异不显著(P>0.05)。因此,该位点有可能作为南阳牛生长性状辅助选择的标记之一。秦川牛和郏县红牛群体中没有发现与该基因座多态显著相关的性状。

In this study,polymorphism of exon10 locus（E10） at the coding region of the bovine Gli3 gene were detected in 708 cattle,and polymorphism was detected at this locus.The fragments showing different SSCP（single strand conformation polymorphism） patterns were sequenced,and a total of two SNPs（NW_001494928：g.205649TC,205754AC） were found.The association analysis of different genotypes with some of the growth and development traits showed that the 24 month-aged individuals with genotype CC had a higher average daily gain than the individuals with genotype TT（P0.05） in Nanyang cattle,while different genotypes had no significant association with growth traits in Qinchuan cattle population and Jiaxian red cattle population.It was suggest that this locus may be suitable to develop molecular markers in the marker-assisted selection（MAS） program for growth traits in Nanyang cattle,but non polymorphism at the locus was detected in Qinchuan cattle population and Jiaxian red cattle population.