β-catenin在藏山羊切牙牙胚发育过程中的表达

Expression of β-catenin during Tibet Goat Incisor Germ Development

研究β-catenin在藏山羊牙胚发育过程中的表达与分布变化,并探讨其在牙齿发育过程中的作用机制。制备藏山羊切牙各期标本,并采用免疫组化方法观察。结果表明,帽状期在星网层、内釉上皮、牙囊均为细胞核阳性表达;钟状早期在内釉上皮、牙乳头细胞核阳性表达;钟状晚期在颈环、牙尖细胞膜阳性表达;成釉期早期在成釉质细胞、成牙本质细胞阳性表达;成釉期晚期均阴性表达。β-catenin在早期牙胚发育中有特定的时空表达模式,通过参与Wnt信号通路和作为黏附分子两种方式,对确定牙胚的形态发生重要作用。

To investigate spatiotemporal expression and variation of β-catenin during tooth germ development on Tibetan Goat and to discuss possible function mechanism in dental development,specimens at different developing stages of Tibetan Goat incisor teeth germ were prepared and immunohistochemical staining was conducted.Results showed that β-catenin was positive expression in stellate reticulum,inner dental epithelium,and dental sac at cup stage,in inner dental epithelium,dental papilla cell,and cell nucleus at early bell stage,in cervical loop and cusp cell membrane at late bell stage,and in ameloblast and odontoblast cell at amelogenesis stage.The expression of β-catenin was spatiotemporal difference in the period of dental development and regulated the teeth germ morphogenesis by means of being as cell adhesion molecular and involving in Wnt signaling pathway.