注射pGRF基因质粒对猪血液生理生化指标和肉品质的影响

Effect of Injecting pGRF Gene Plasmid on Biochemical Indicators in Blood and Meat Quality of the Growth Pigs

通过检测注射pGRF基因质粒(pig growth hormone-releasing factor gene plasmid)后生长猪血液生化指标与肌肉品质指标,探讨该制剂对生长猪的健康水平、肉品质和安全性的影响。将8头二元杂交、60日龄左右的阉后公猪随机分为两组,一组注射pGRF基因质粒,一组注射等量生理盐水,进行了60 d对比试验,于处理后第7、14、28、56天早晨空腹采血制备血清,测定生化指标。结果表明:注射pGRF基因质粒后第14天,除血清尿素氮提高39.88%外,血糖、尿素氮、肌酐、甘油三酯、胆固醇浓度变化均不显著。由此可见,注射pGRF基因质粒后,除血清尿素氮升高外,生长猪的其他生化指标都没有明显变化。于处理后第60天,每组随机选3头屠宰,称量器官质量,并采样测定肉品质。与对照组相比,试验组猪肉剪切力下降7.28%(P>0.05),滴水损失降低20.86%(P>0.05),而熟肉率提高4.38%(P>0.05);但肌间脂肪比对照组提高68.83%(P<0.05);肉色a*值降低14.30%(P>0.05),b*、L*值有所提高,但差异均未达到显著水平;pGRF基因质粒对胴体pH影响不明显,器官与胴体的比值差异均未达显著水平(P>0.05),其中从平均值看心脏质量比例略有增加,脾、肾比例有所下降。从本试验结果看,注射pGRF基因质粒对各项猪肉品质指标无不良影响,还可改善肌肉嫩度,降低滴水损失和提高肌间脂肪含量。

The concentrations of biochemical indicators in the serum,and muscle indicators of growth pigs injected with pGRF gene plasmid（pig growth hormone-releasing factor gene plasmid） were detected to explore the effects of this preparation on healthy level,quality and safety of pork.Eight castrated boar pigs with similar weight（15 kg±0.43 kg） crossbred from Landrace×Large white were allocated into treatment and control group.The treatment group was injected with 2 mL（i.e.4.5 mg） gene plasmid into neck once,and the control group was done in the same way with 2 mL normal saline.These groups were compared during the 60 days test.Serums were prepared to determine the biochemical indicators at 7th-,14th-,28th-and 56th-day after treatment.The results showed that the glucose,creatinine,triglyceride and serum cholesterol concentrations were no significant difference between two groups except that urea nitrogen of treatment group was 39.88% more than that of control group（P0.05） at day 14.These results showed that the biochemical compositions in serum of growth pigs were not affected by pGRF gene plasmid injected but urea nitrogen.At 60th-day after injecting pGRF gene plasmid,three pigs were randomly selected from each group to measure slaughter weight,organ weight and pork quality.Compared with control group,the shear force and drip loss of pork dropped separately by 7.28% and 20.86%（P0.05）,the rate of cooked meat was increased by 4.38%（P0.05）,but intramuscular fat increased by 68.83%（P0.05）.The color a values reduced by 14.30%（P 0.05）,b and L values increased,but the difference was not reach a significant level.pGRF gene plasmid had little effect on pH of the carcass.There was no significant difference on ratio of organs to carcass between two groups（P0.05）.The ratio between heart and carcass increased slightly,and the ratios of spleen and kidney were declined,but both of them were in the normal range.In short,injecting the pGRF genes plasmid has no adverse effects on the quality of carcass.