摘要
从140对SSR引物中筛选出13对多态性好的引物,构建了14份材料的DNA指纹图谱,筛选出青杂2号、青杂5号共显性SSR标记,检测了青杂2号、青杂5号大田抽样F1群体L15、L41的纯度。结果表明SSR标记鉴定检测方法比大田检测更快更准确。
The aim of this research was to construct fingerprints of 14 materials Qingza hybrid lines(brassica napus) and its parents using 13 SSR primers selected from 140 SSR primers.Codominance SSR markers were found in Qingza No.2,Qingza No.5 and their parents.Those primers were used to test the purity of Qingza No.2 hybrid and Qingza No.5 hybrid in fields.The results indicated that SSR marker was an efficient tool to detect false hybrid,compared with field observation method.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第3期81-86,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家"863"项目(2009AA101105)
农业科技成果转化资金项目(2007GBZG200337)