春性双低甘蓝型油菜杂交种和亲本指纹图谱构建及杂交种纯度鉴定

Fingerprint Construction of Hybrids and Related Parents in Brassica napus and Hybrid Purity Test

从140对SSR引物中筛选出13对多态性好的引物,构建了14份材料的DNA指纹图谱,筛选出青杂2号、青杂5号共显性SSR标记,检测了青杂2号、青杂5号大田抽样F1群体L15、L41的纯度。结果表明SSR标记鉴定检测方法比大田检测更快更准确。

The aim of this research was to construct fingerprints of 14 materials Qingza hybrid lines（brassica napus） and its parents using 13 SSR primers selected from 140 SSR primers.Codominance SSR markers were found in Qingza No.2,Qingza No.5 and their parents.Those primers were used to test the purity of Qingza No.2 hybrid and Qingza No.5 hybrid in fields.The results indicated that SSR marker was an efficient tool to detect false hybrid,compared with field observation method.