美女樱(Verbena hybrida)茎尖组织培养

Tissue Culture and Rapid Propagation of Verbena (Verbena hybrida)

以2个美女樱品种的茎尖为外植体,筛选适宜的消毒方法和初代培养、继代培养及生根培养的最佳培养基,建立了现代美女樱优良品种的离体快繁体系。结果表明,外植体消毒以70%酒精浸泡15 s、升汞浸泡4 min为宜,污染率较低（10%）,萌发率很高（87.5%）。供试的2个美女樱品种初代萌发和继代培养对不同激素浓度配比有一定差异。大花品种Quartz的适宜继代培养基为MS＋BA1.0 mg/L＋NAA0.2 mg/L,小花品种Aztec的适宜继代培养基为MS＋BA0.5 mg/L＋NAA0.1 mg/L。对于同一品种而言,较高浓度6-BA可以直接从叶上诱导出愈伤组织,并由愈伤组织形成少量不定芽。2个品种在1/2MS＋NAA0.05 mg/L培养基上均能生根,生根率达96%~98%。

Using stem tips of two verbena cultivars as explants,the tissue culture and rapid propagation system of modern verbena cultivars was established on the base of screening suitable disinfection method,and proper culture mediums for regeneration.The results indicated that the most effective disinfection method was to soak explants with 70% alcohol for 15 s and 0.1% HgCl2 for 4 min,in which the lower contamination rate reached to 10% and maximum surviving rate to 87.5%.Concerning the different hormone concentration requirements of induction and subculture,obvious genotype difference could be observed.The optical subculture medium for big flower Quartz and small flower Aztec was MS＋BA 1.0 mg/L＋NAA 0.2 mg/L and MS＋BA0.5 mg/L＋NAA0.1 mg/L respectively.For a given cultivar,high concentration of 6-BA might induce calluses from leaf directly,at the same time,a few adventitious buds were gained from the calluses.Root of both cultivars was easily induced on 1/2MS medium supplemented with 0.05 mg/L NAA,and rooting rate of 96%~98% could be obtained.