摘要
目的:研究黄芪双向性固体发酵前后黄芪甲苷的转化。方法:采用HPLC测定法、柱色谱分离法、波谱鉴定技术进行发酵前后的成分变化研究;观察转化产物对血管内皮细胞的抗氧化效应。结果:在黄芪发酵过程中,黄芪甲苷转化形成6-O-β-D-葡萄糖基-环黄芪醇,该转化物具有显著的抗氧化效应。结论:黄芪中有效成分黄芪甲苷经药用真菌(灵芝)双向性固体发酵后发生了生物转化,这一转化具有增效的作用。
Objective:To study the transformation of Astragaloside IV in bidirectional solid fermenting of Astragalus membranaceus var.mongholicus.Methods:The chemical components of pre and post-fermenting were comparatively analyzed by techniques of HPLC,column chromatography and spectral identification.Antioxidant effect of transformational product on vascular endothelial cells was researched.Results:In fermenting of Astragalus membranaceus,Astragaloside IV was transformated into 6-O-β-D-glucoside cycloastragenol,which had significant antioxidant effect.Conclusion:Biotransformation of Astragaloside IV in Astragalus membranaceus was occurred after bidirectional solid fermenting with Ganoderma lucidum,and the biotransformation could enhance effect.
出处
《中药材》
CAS
CSCD
北大核心
2010年第3期339-343,共5页
Journal of Chinese Medicinal Materials
基金
江苏省教育厅项目(JH07-17)
南京市科技局项目(2008创基030)
南京晓庄学院重点研究项目(20007NXY16)
关键词
灵芝
黄芪
黄芪甲苷
双向性固体发酵
6-O-β-D-葡萄糖基-环黄芪醇
Ganoderma lucidum(Curtis:Fr.)P.Karst.
Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao
Astragaloside IV
Bidirectional solid fermentation
6-O-β-D-glucoside cycloastragenol