摘要
目的:研究黄芪双向性固体发酵前后黄芪甲苷的转化。方法:采用HPLC测定法、柱色谱分离法、波谱鉴定技术进行发酵前后的成分变化研究;观察转化产物对血管内皮细胞的抗氧化效应。结果:在黄芪发酵过程中,黄芪甲苷转化形成6-O-β-D-葡萄糖基-环黄芪醇,该转化物具有显著的抗氧化效应。结论:黄芪中有效成分黄芪甲苷经药用真菌(灵芝)双向性固体发酵后发生了生物转化,这一转化具有增效的作用。
Objective：To study the transformation of Astragaloside IV in bidirectional solid fermenting of Astragalus membranaceus var.mongholicus.Methods：The chemical components of pre and post-fermenting were comparatively analyzed by techniques of HPLC,column chromatography and spectral identification.Antioxidant effect of transformational product on vascular endothelial cells was researched.Results：In fermenting of Astragalus membranaceus,Astragaloside IV was transformated into 6-O-β-D-glucoside cycloastragenol,which had significant antioxidant effect.Conclusion：Biotransformation of Astragaloside IV in Astragalus membranaceus was occurred after bidirectional solid fermenting with Ganoderma lucidum,and the biotransformation could enhance effect.
出处
《中药材》
CAS
CSCD
北大核心
2010年第3期339-343,共5页
Journal of Chinese Medicinal Materials
基金
江苏省教育厅项目(JH07-17)
南京市科技局项目(2008创基030)
南京晓庄学院重点研究项目(20007NXY16)
关键词
灵芝
黄芪
黄芪甲苷
双向性固体发酵
6-O-β-D-葡萄糖基-环黄芪醇
Ganoderma lucidum（Curtis：Fr.）P.Karst.
Astragalus membranaceus（Fisch.） Bge.var.mongholicus（Bge.） Hsiao
Astragaloside IV
Bidirectional solid fermentation
6-O-β-D-glucoside cycloastragenol
