青蒿素对大鼠肾小球系膜细胞增殖、凋亡及Caspase-3活性的影响

Effects of Artemisinin on Proliferation,Apoptosis and Caspase-3 Expression of Rat Mesangial Cell

目的:观察青蒿素对大鼠肾小球系膜细胞(mesangial cell,MC)增殖、凋亡及Caspase-3活性的影响。方法:以体外培养大鼠肾小球系膜细胞为研究对象,应用不同质量浓度青蒿素干预系膜细胞,分别采用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法、荧光倒置显微镜及酶标分析仪检测系膜细胞的增殖、凋亡及Caspase-3活性。结果:与对照组比较,不同质量浓度青蒿素对肾小球系膜细胞的增殖及Caspase-3活性均有不同程度的影响(P<0.01);与地塞米松组比较,其他2组相同质量浓度药物对肾小球系膜细胞增殖及Caspase-3活性均有显著影响(P<0.01),尤以青蒿素联合地塞米松高剂量组的抑制作用最强,且3组药物对肾小球系膜细胞的增殖、Caspase-3活性及其凋亡的影响均呈剂量依赖趋势,在中、高剂量药物联合治疗组中出现了系膜细胞大量死亡。结论:青蒿素能剂量依赖性抑制系膜细胞增殖、增强Caspase-3活性及促进肾小球系膜细胞凋亡。

Objective：To study the effects of artemisinin on proliferation,apoptosis and Caspase-3 active of rat mesangial cell.Methods：Rat mesangial cells were incubated with different concentrations of artemisinin,the proliferation,apoptosis and Caspase-3 active of rat mesangial cell were measured by MTT assay,fluorescent inverted microscope and enzyme-labeled analytical instruments respectively.Results：Compared with control group,the proliferation and Caspase-3 expression of mesangial cell of three other groups were sinificantly different（P〈0.01）.Compared with dexamethasone group,there were significant difference effects of proliferation and Caspase-3 expression of mesangial cell in other two groups of identical concentration drugs（P〈0.01）,especially in the artemisinin＋glucocorticoid（ArtGC） group,and the effects of three different drugs on the mesangial cell Caspase-3 expression,proliferation and apoptosis had the tendency of depend on dosage,and mass mortality of mesangial cell in the mediate-dosage and high-dosage ArtGC group.Conclusion：Artemisinin could inhibit the proliferation of mesangial cell,enhance the expression of Caspase-3 and promote the apoptosis in a dose-dependent manner.