盾叶薯蓣提取物对乳鼠心肌细胞缺血/再灌注损伤的保护作用

Protective Effect of Extractive of Dioscorea zingiberensis against Ischemia/Reperfusion Injury in Cultured Neonatal Rat Cardiomyocytes

目的:确定从盾叶薯蓣中提取、分离得到的一水溶性化合物(SY-A)的结构,并研究其对乳鼠心肌细胞缺血/再灌注(I/R)损伤的保护作用。方法:利用柱层析技术从盾叶薯蓣中分离到水溶性化合物SY-A,经1H-NMR、13C-NMR、135DEPT鉴定其结构;采用体外培养乳鼠心肌细胞,建立心肌缺血/再灌注损伤模型。比较正常对照组、模型组、SY-A各组(50、100、150 mg/L)培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)含量变化,MTT法检测细胞存活率。结果:SY-A结构为4-甲氧基-苯甲酸-2-O-葡萄糖苷;与I/R组比较,SY-A组心肌细胞LDH、CK的漏出量显著降低,细胞存活率显著提高。结论:盾叶薯蓣中水溶性成分SY-A对乳鼠心肌细胞缺血/再灌注损伤有显著的保护作用。

Objective：To define the structure of a water-soluble compound（SY-A） extroeted from Dioscorea zingiberensis and investigate its protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion（I/R） injured.Methods：The SY-A was isolated from Dioscorea zingiberensis by utilizing column chromatograph and its structure was identified by ^1H-NMR,^13C-NMR,^135DEPT.Neonatal rat cardiomyocytes were cultured and divided into 5 groups randomly.Then a model of ischemic/reperfusion（I/R） was established.Cells were divided into control group,simulated ischemia/reperfusion group（I/R group）,I/R＋SY-A（50 mg/L） group,I/R＋SY-A（100 mg/L） group and I/R＋SY-A（150 mg/L）group.The levels of malondialdehyde and Creatine Kinase were measured.The cell viability was determined by MTT assay.Results：The structure of SY-A was identified as 4-methoxybenzoic acid-2-O-glucopyranoside.Compared with I/R group,the levels of LDH and CK were decreased sinificantly in the SY-A group,and the cell viability was increased obviously.Conclusion：Extractive of Dioscorea zingiberensis showed protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion injured.