摘要
L-异亮氨酸是L-亮氨酸发酵的主要副产物,L-异亮氨酸的积累对L-亮氨酸的发酵及提取均产生不利影响。采用基因重组技术敲除L-亮氨酸产生菌-谷氨酸棒杆菌(Corynebacterium glutamicum)TGL8207的苏氨酸脱氨酶基因ilvA,构建了ilvA缺失株谷氨酸棒杆菌(C.glutamicum)TGL8207 ilvA。发酵结果显示:ilvA基因缺失对TGL8207 ilvA的生长影响很小。与供试菌比较,TGL8207 ilvA的L-亮氨酸产量和糖酸转化率分别提高了8.4%和1.9%。
L-isoleucine is the main by-product in L-leucine fermentation.The accumulation of L-isoleucine has a negative impact on L-leucine fermentation and retrieval.In this study,threonine deaminase gene ilvA of the L-leucine producing train C.glutamicum TGL8207 was knocked out using gene recombination technology and a ilvA deletion mutant C.glutamicum TGL8207 ilvA was constructed.The fermentation result indicated that ilvA gene deletion has little effect on the growth of TGL8207 ilvA.Compared with the original strain,the L-leucine yield and sugar/acid conversion rate of TGL8207 ilvA were increased by 8.4% and 1.9%,respectively.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2010年第2期40-45,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家863计划项目(2007AA02Z200)
天津市应用基础及前沿技术研究计划(08JCZDJC15400)
关键词
谷氨酸棒杆菌
L-亮氨酸
苏氨酸脱氨酶
基因敲除
发酵
Corynebacterium glutamicum
L-leucine
threonine deaminase
gene knockout
fermentation
