阿魏对阿魏菇子实体抗氧化性能及氨基酸组成的影响

Influence of Asafoetida on the Antioxygenic Property and Amino Acid Composition of Asafoetida Mushroom Fruiting Body

通过在培养基中添加不同剂量的阿魏粉,培养获得阿魏菇子实体。对阿魏菇提取物的DPPH.自由基清除率及氨基酸组成进行分析,结果表明,添加阿魏粉的阿魏菇,3种溶剂提取物的DPPH.自由基清除率均高于CK,且呈一定的量效关系。以水提物的自由基清除效果最好,75%乙醇提取物次之,石油醚提取物最差。添加9%阿魏粉的阿魏菇的总氨基酸、必需氨基酸含量均最高,分别为27.42 g/100 g和13.6 g/100 g;其次是野生阿魏菇,分别为9.61 g/100 g和23.59 g/100 g;CK最低,分别为8.9 g/100 g和20.61 g/100 g。阿魏菇氨基酸化学评分:以添加9%阿魏粉的评分最高,其次是CK,野生阿魏菇最低;阿魏菇的蛋白质氨基酸评分以CK最高,其次是添加9%阿魏粉的,野生阿魏菇的评分最低。说明阿魏粉对阿魏菇子实体抗氧化性能及氨基酸组成均有较大影响。

In this experiment,the Asafoetida mushroom fruiting body was cultured and obtained by adding different doses of Asafoetida powder to the medium.The DPPH.scavenging rate of the fruiting body extractions and amino acid compositions of the Asafoetida mushroom were also analyzed.It was shown in the results：By adding Asafoetida powder cultivation,the Asafoetida mushroom extraction obtained from three different solutions showed good free radical scavenging rates.The DPPH.scavenging rates were all higher than CK samples,and it showed some dose-effect relationship：the water extract was better than the alcohol extract,and the petroleum ether extract was the lowest.The contents of total amino acids and essential amino acid were all the highest in the Asafoetida mushroom cultured by adding Asafoetida powder,they were 27.42 g/100 g and 13.6 g/100 g,respectively.Followed by the wild asafoetida mushroom and the contents were 9.61 g/100 g and 23.59 g/100 g,respectively.It was the lowest in the CK sample.They were 8.9 g/100 g and 20.61 g/100 g.The chemical score was in the order of adding Asafoetida powder（9%） cultured Asafoetida mushroom,CK sample and wild Asafoetida mushroom.The amino acid score is in the order of CK sample,adding 9% Asafoetida powder sample and the wild Asafoetida mushroom.It was shown that Asafetida has great influence on the antioxiygenic prop-erty and amino acid composition of Asafoetida mushroom fruiting body.