蓝靛果花色苷超声波辅助提取优化及其降血脂作用

Optimization on the Supersonic-assisted Extracting Technology of Lonicera Caerulea Anthocyanin and Its Hypolipidemic Effect

以蓝靛果果渣为原料,采用超声波辅助法提取蓝靛果果渣中的花色苷(LCRA)。用响应面分析法研究了乙醇体积分数、超声波功率、液固比、提取时间4个因子对花色苷提取效果的影响。此外还研究了蓝靛果花色苷对高血脂大鼠的降血脂作用。用SAS软件确定的最佳提取工艺条件:乙醇体积分数70.7%、超声波功率302.2W,提取时间6.4 min,液固比20.2。在此条件下花色苷提取率为84.8%。动物试验表明,与模型组相比,蓝靛果花色苷可降低高脂血症大鼠血脂水平,提高抗动脉硬化指数(AAI),使肝脏LPS、HL、LPL酶活性明显增强。蓝靛果果渣花色苷具有降低血脂及预防动脉粥样硬化作用。

Extracting anthocyanin from Lonicera caerulea residue by Supersonic-assisted technique.The influence of ethanol concentration,supersonic power,the ratio of solution to solid and extraction time on the extraction and the hypolipidemic effects of anthocyanin were studied.The optimal extraction conditions were determined by SAS software as follows,the ethanol concentration 70.7%,Supersonic power 302.2W and the extraction time 6.4 min,ratio of solution to solid 20.2,the extraction rate was 84.8%.The animal experimentation indicated that the levels of serum lipids in hyperlipidemic rats fed with anthocyanin were significantly lower and AAI was obviously increased than those in model control group,the activities of HL,LPL,LPS in liver were increased in anthocyanin groups.Anthocyanin from Lonicera caerulea residue has significantly hypolipidemic and anti-artherosclerosis effects.