瑞舒伐他汀和阿托伐他汀对新生大鼠心脏成纤维细胞增殖和胶原合成的作用

Comparative effects between rosuvastatin and atorvatatin on cardiac fibroblasts proliferation and collagen synthesis of neonatal rat

目的比较瑞舒伐他汀和阿托伐他汀对新生SD大鼠心脏成纤维细胞(CFs)的抑制作用。方法采用新生SD大鼠CFs进行体外培养,分别给予不同剂量瑞舒伐他汀和阿托伐他汀进行干预。应用MTT法测定CFs细胞数,5-溴脱氧尿嘧啶法测定DNA合成,羟脯氨酸法测定胶原合成,流式细胞仪测定细胞增殖周期。结果瑞舒伐他汀和阿托伐他汀均可明显抑制新生SD大鼠CFs增殖、DNA合成和胶原合成。在相同体外药物浓度下,2药对成纤维细胞的抑制作用无明显差异。2药均可增加CFs处于G0/G1期的百分率,降低CFs处于S期的百分率。结论瑞舒伐他汀和阿托伐他汀均为有效的抗心脏纤维化药物,2种药对心脏成纤维细胞的抑制作用无明显差异。

AIM To compare the effects of rosuvastatin and atorvastatin on cardiac fibroblasts （CFs） proliferation, DNA synthesis, and collagen synthesis of neonatal rat. METHODS CFs of neonatal Sprague- Dawley rats were isolated and treated with rosuvastatin or atorvastatin, respectively. MTT assay, Brdu staining, hydroxyproline method, and flow cytometry technology were used to compare the inhibition effects of the two drugs. RESULTS Both rosuvastatin and atorvastatin inhibited CFs proliferation, DNA synthesis and collagen synthesis markedly. The inhibitory effects on CFs were similar between the two drugs at the same concentration. Both of them increased the percent of CFs in G0/G1 phase, and decreased the percent of CFs in S phase. CONCLUSION Both rosuvastatin and atorvastatin are effective antifibrotic agents, and their effects are equal to each other.