中药何首乌饮对衰老大鼠卵巢组织形态学影响的实验研究

Effect of the traditional Chinese medicine Heshouwuyin on ovary morphology in aging rats

目的:观察中药何首乌饮对衰老模型大鼠卵巢形态学的影响。方法:将48只SD大鼠随机分为3组:正常组、模型组、预防组。采用D-半乳糖连续腹腔注射法制造雌性大鼠亚急性衰老模型,预防组在造模的同时以中药方剂何首乌饮灌胃。应用HE染色及透射电镜方法观察各组大鼠卵巢组织形态学的变化。结果:何首乌饮明显增加了衰老大鼠卵巢组织中原始卵泡、生长卵泡及黄体的数量(P<0.05,P<0.01),且明显改善了衰老大鼠卵巢颗粒细胞的超微结构,使滑面内质网增加,线粒体及高尔基复合体丰富。结论:何首乌饮通过影响衰老大鼠卵巢形态学的改变,具有预防和延缓颗粒细胞凋亡而达到阻止卵泡闭锁,可能是抗大鼠性腺衰老的作用机制之一。

Objective： To observe the ovary morphological changes in aging rats after treatment with the traditional Chinese medicine Heshouwuyin（HSWY）.Methods： Rats（n=48） were randomly divided into 3 groups： normal,aging control and HSWY groups.Subacute aging model was induced by intraperioneal injection of D-galactose.The rats in HSWY group were administered with various doses of intragastric HSWY during D-galactose injection.The ovary morphology was observed by hematoxylin-eosin staining and transmission electron microscopy.Results： HSWY significantly increased the number of primordial follicles,growing follicles and corpus luteums（P0.05 or P0.01）.HSWY improved the substructures of the granular cells,and increased the smooth endoplasmic reticulum,mitochondria and Golgi complex.Conclusion： HSWY can regulate the morphology of the ovary and prevent the apoptosis of granular cells,suggesting an anti-aging ability in rat sexual gland.