利钠肽C受体在利钠肽舒张猪冠状动脉血管中的作用

Effect of C-Type Natriuretic Peptide Receptor in Swine Coronary Artery Dilation

目的:探讨利钠肽C受体是否参与3种利钠肽的舒张血管作用。方法:采用离体血管环灌流方法,观察心房利钠肽(ANP)、脑钠肽(BNP)、C型利钠肽(CNP)、硝酸甘油和利钠肽C受体激动剂cANF4-23对猪冠状动脉的作用;然后加入利钠肽C受体阻断剂cANF4-28观察利钠肽舒张血管作用的变化。实验分为8组(n分别=8):分别为ANP组、BNP组、CNP组、硝酸甘油组、cANF4-23组、cANF4-28+ANP组、cANF4-28+BNP组和cANF4-28+CNP组。结果:CNP组冠状动脉最大舒张率(36.5±4.0)%低于ANP组(60.4±22.6)%、BNP组(68.5±11.5)%和硝酸甘油组(53.8±6.9)%,差异均有统计学意义(P均<0.05);其余各组间比较差异无统计学意义(P均>0.05)。cANF4-28+CNP组最大舒张率为(21.3±10.3)%与CNP组(36.5±4.0)%比较冠状动脉最大舒张率明显减弱(P<0.05),差异有统计学意义。cANF4-23组冠状动脉最大舒张率为(42.4±17.6)%,与CNP组相比差异无统计学意义(P>0.05)。结论:3种利钠肽对猪冠状动脉均有良好的扩张作用;利钠肽C受体参与了CNP对猪冠状动脉的舒张作用,而没有参与ANP和BNP的扩张冠状动脉作用。

Objective：To investigate the possible involvement of C-type natriuretic peptide receptor（NPR-C） on swine coronary artery di- lation mediated by three kinds of natriuretic peptides（NPs）. Methods：In vitro pharmacological investigation was conducted in isolated vascular ring from swine coronary artery. The experiment was designed into 8 groups. Atrial natriuretic peptide （ANP group） , brain natriuretic peptide（ BNP group）, C-type natriuretic peptide （ CNP group ）, nitroglycerin （ NTG group ） ; NPR-C agonist cANF4-23 group ; in addition, NPR-C antagonist cANF4-28 was used as cANF4-28 ＋ ANP group, cANF4-28 ＋ BNP group and cANF4-28 ＋ CNP group. The relaxant effects of three NPs and NTG were examined,the effect of NPR-C ＂agonist cANF4-23 was observed and the effects of NPs with cANF4-28 were evaluated on swine coronary artery respectively. Resuhs ：Vasorelaxant activities of ANP, BNP,CNP andNTG were（60. 4 ±22. 6）%, （68. 5 ± 11.5）%, （36. 5 ±4. 0）% ,and （53.8 ± 6. 9）%, respectively, relaxant response of swine coronary artery to CNP was lower than the others, （P 〈 0. 01 respectively）. NPR-C antagonist could inhibit the vasorelaxant activity of CNP. NPR-C agonist could produce the similar relaxant activity of CNP. Conclusion ： Three kinds of NPs presented significant vasodilatory effects on swine coronary artery. NPR-C was involved in the effect of CNP dilation of swine coronary artery.