过滤染色法检查精子浓度的方法学研究

Filtering-staining method for measuring sperm concentration

目的:开发一种快速精子浓度检测方法,并评价其可行性。方法:根据精子可被亚甲兰溶液染成蓝色,其颜色深浅与精子数量呈正相关原理,以经显微镜计数确认浓度的精子(2000万个/ml)为标准样本,加入亚甲蓝溶液染色,过滤,根据结果颜色,应用现代印刷技术制备出标准色标。以颜色深浅检验标本中精子浓度,对方法中涉及的过滤膜、染色液、染色时间、标准色标等关键参数进行确认,评价试剂盒检测结果。结果:通过对3000份成年男性精液样品检测结果表明,该试剂盒方法的灵敏度、特异性和准确性均在95%以上。结论:本方法所制试剂盒能够实现快速自检,适合医院检查前初步诊断,时间短,操作简单。

Objective：To develop a rapid self-testing method for measuring sperm concentration and determine the feasibility of this method.Methods：The sperm cells could be dyed by methylene blue solution,and the color gradation associated with the sperm number.The sperm samples with the concentration of 20 million /ml set as standard samples were dyed with a prepared methylene blue solution,and filtered to remove the unbound dye.The average code developed from the color gradation of the standard samples served as a standardized color code.The key parameters of this method including the filtering membrane,the staining solution,the duration of staining,and the standardized color code were explored.Results：Verified with 3 000 samples donated by adult male volunteers,the sensitivity,specificity and accuracy of this method were found to be more than 95%.Conclusion：This method with short testing duration and easy operation can be used for rapidly self-test and appropriate for primary diagnosis at home.