受烯丙异噻唑诱导的水稻叶片蛋白质表达差异分析

Analysis of expressed protein differences in rice leaves induced by pobenazole

采用烯丙异噻唑对空育131水稻进行灌根处理,利用双向电泳技术分析诱导后的水稻叶片和对照叶片蛋白质的表达差异,通过PDQuest8.0.1软件分析比较诱导组和对照组的双向电泳图谱后找到10个差异表达(2倍以上)上调的蛋白质点,取其中表达量较大的8个进行质谱分析。结果显示,这8个蛋白分别具有泛醌-细胞色素C还原酶活性、苏氨酸肽链内切酶活性、苹果酸脱氢酶活性、甘油醛-3-磷酸脱氢酶活性、磷酸核酮糖羧化酶活性、交替氧化酶活性、抗坏血酸过氧化物酶活性和质体特异性30S核糖体蛋白质活性。其中P1、P3和P4蛋白参与植物的呼吸代谢途径;P2蛋白参与蛋白质降解途径;P5蛋白参与植物糖的合成;P6和P7蛋白参与活性氧代谢平衡。研究表明,烯丙异噻唑是通过增强水稻呼吸作用、激活蛋白质降解、促进抗病相关糖的合成和提高活性氧清除能力等从而提高水稻的抗病性。

Rice variety Kongyu 131 was treated with pobenazole by root-irrigation, and the expressed proteins of rice leaves between the induced group and the concurrent control were analyzed using two-dimensional electrophoresis （2-DE）. Through comparison of 2-DE maps between the two groups which was analyzed by PDQuest 8.0.1, at least ten up-regulated proteins （ over two times than the concurrent control） were obtained, eight of them were identified with mass spectrometry. It was shown that they were well characterized including ubiquinol-cytochrome creductase, threonine endopeptidase, malate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, ribulose bisphosphate carboxylase, alternative oxidase, aseorbate peroxidase and plastid-specific 30S ribosomal proteins. Of which, the P1, P3 and P4 protein participate in respiration metabolism of plants ; P2 protein might participate in degradation of protein; P5 protein could participate in synthesis of sugar; P6 and P7 protein participate in regulation balance of active oxygen metabolism. Analysis of differential proteins showed that probenazole induced rice resistance through increasing respiration of rice, degrading the proteins, promoting synthesis of resistanceassociated sugar and scavenging of active oxygen.