番茄叶霉病菌不同生理小种毒素的分离纯化与生物测定

Isolation,purification and bioassay of toxin proteins secreted by different physiological races of tomato leaf mould pathogen, Cladosporium fulvum

采用凝胶过滤方法对番茄叶霉病菌6个生理小种所产生的毒素进行了分离、纯化,并利用叶片针刺法分别对其蛋白组分进行了生物活性测定,以探讨番茄叶霉病菌不同生理小种毒素组分对番茄叶片的致病力的影响。结果表明,每个生理小种毒素的纯化峰蛋白组分中只有一个蛋白组分能使番茄叶片褪绿,分别为来自生理小种1.2.3的第4个峰蛋白组分(记作:1.2.3(4),下同)、1.2.4(2)、1.2.3.4(2)、1.3(2)、1.3.4(2)和1.4(2)。说明这几个峰蛋白组分对番茄叶片有一定的致病力,而其它峰蛋白组分对番茄叶片则没有明显致病力。透射电子显微镜观察结果显示,叶绿体膨胀或变形、基质片层部分消解,其中1.2.3.4(2)处理的叶绿体解体最彻底。

To explore the virulence effect of all peaks＇ protein compositions from different physiological races to tomato leaf, gel filter with liquid chromatography methods was used to purify toxin proteins secreted by six physiological races of tomato leaf mould pathogen and the bioassay was carried out by using needling leaf method. The results showed that only one peaks＇protein compositions secreted by each physiological race could make the leaf chlorosis. These peaks＇ protein compositions were from the fourth peaks＇ protein compositions of physiological race 1.2.3 （ denoted 1.2.3 （4） , and the following analogy）, 1.2.4 （2）, 1.2.3.4 （2）, 1.3 （2）, 1.3.4 （2）, 1.4 （2）. The cell uhrastructure of chlorosis tomato leaf observed under transmission electron microscope, and we found that chloroplasts were relaxed and swollen, and chloroplast grana were dissolved, especially treated by physiological race 1.2.3.4 （ 2 ） peak＇ protein.