大豆异黄酮抵抗体外培养猪脂肪细胞氧化损伤的作用

The Propective Effects of Soybean Isoflavones against Oxidative Damage to Porcine Adipocytes in vitro

为了探明大豆异黄酮对猪脂肪细胞氧化损伤的保护效应,试验分别用含0、10、20、40μmol/L和80μmol/L异黄酮S(一种合成的大豆异黄酮)或三羟基异黄酮(GEN)的完全培养液培养猪脂肪细胞48h,用终浓度为100μmol/L的FeSO4和H2O2溶液进行氧化处理1 h。结果表明:与正常对照组相比,氧化对照组细胞内活性氧(ROS)水平和脂质过氧化产物丙二醛(MDA)含量分别升高了1.61倍和2.74倍(P<0.01);与氧化对照组相比,添加10、20、40μmol/L和80μmol/L异黄酮S使细胞内ROS水平分别下降了24.62%(P<0.05)、20.03%(P<0.05)、37.88%(P<0.01)和41.20%(P<0.01);添加10、20、40μmol/L和80μmol/L GEN均显著降低了细胞内ROS水平(P<0.01)和MDA含量(P<0.05)。试验结果提示在本试验条件下,异黄酮S和GEN能通过抑制猪脂肪细胞的脂质过氧化、降低ROS产生,抵抗羟自由基对猪脂肪细胞的氧化损伤。

Porcine adipocytes were exposed to a synthetic isoflavone（ISO-S）or genistein（GEN） at different concentrations（0,10,20,40 and 80 μmol/L） for 48 h,respectively.Then the medium was changed to one containing 100 μmol/L FeSO4 and H2O2.Cells were incubated for another 1 h before sampling to evaluate the protective effects of soybean isoflavones against oxidative damage to adipocytes.Two-factorial ANOVA revealed that reactive oxygen species（ROS） levels and malondialdehyde（MDA） contents in porcine adipocytes treated by Fe2＋/H2O2 system were influenced by the concentrations of soybean isoflavone（P〈 0.05）.Compared to the control cells,the cellular levels of ROS and MDA in oxidized control group were increased by 1.61 times（P〈 0.01） and 2.74 times（P 〈0.01）,respectively.When adipocytes were preincubated with 10,20,40 and 80 μmol/L ISO-S,the cellular level of ROS was decreased by 24.62%（P〈 0.05）,20.03%（P〈 0.05）,37.88%（P 〈0.01） and 41.20%（P〈 0.01） respectively,with respect to the oxidized control.Preincubation with GEN（10,20,40 and 80 μmol/L） significantly decreased the levels of ROS（P〈 0.01） and MDA（P〈 0.05） in adipocytes,compared with the oxidized control.These results indicated that preincubation with ISO-S or GEN could alleviate oxidative injury of porcine adipocytes induced by hydroxyl radical through decreasing lipid peroxidation and ROS production.