结直肠癌患者抗肿瘤免疫参数的比较

Comparison of anti-tumor immune parameters in patients with colorectal cancer

目的比较总淋巴细胞计数、CD4+CD25+Treg细胞、Th/Tr淋巴细胞比值、CD4/CD8比值评估结直肠癌患者抗肿瘤免疫状态的意义。方法应用流式细胞术检测60例结直肠癌患者和30例健康者外周血Th/Tr比值、CD4/CD8比值,计算CD4+CD25+Treg计数、淋巴细胞总数,并与30例健康志愿者进行比较。结果结直肠癌患者总淋巴细胞计数较健康志愿者减少,CD4+CD25+Treg较健康志愿者增多(P<0.05);有转移与无转移的结直肠癌患者总淋巴细胞计数、CD4+CD25+Treg细胞计数分别为1478±349vs1576±369(P>0.05)、121±47vs107±64(P>0.05);结直肠癌患者Th/Tr比值小于健康志愿者(P<0.01),有转移结直肠癌患者Th/Tr比值小于无转移患者(P<0.01);CD4/CD8比值在患者与健康志愿者间无统计学差异(P>0.05),且与有无转移无关。结论Th/Tr比值对结直肠癌患者抗肿瘤免疫状态的评估可能优于总淋巴细胞计数、CD4+CD25+Treg细胞计数和CD4/CD8比值。

Objective To evaluate the significance of anti-tumor immune status in patients with colorectal cancer by comparing the number of lymphocytes and CD4＋CD25＋Treg cells,and the Th/Tr and CD4/CD8 value.Methods The Th/Tr and CD4/CD8 value,the number of lymphocytes and CD4＋CD25＋Treg cells in peripheral blood from 60 colorectal cancer patients were detected by flow cytometry and compared with those detected in 30 healthy persons.Results The number of total lymphocytes was smaller while the number of CD4＋CD25＋Treg cells was significantly greater in healthy donors than in colorectal cancer patients（1 478±349 vs 1 576±369 and 121±47 vs 107±64,P0.05）.The Th/Tr value was lower in colorectal cancer patients than in healthy donors and in those without metastasis（P0.01）.No significant difference was found in CD4/CD8 value between patients and healthy donors（P0.05）and the CD4/CD8 value was not related with metastasis.Conclusion The Th/Tr value is advantageous over the number of total lymphocytes and CD4＋CD25＋Treg cells and the CD4/CD8 value in evaluating the anti-tumor immune status in colorectal cancer patients.