小剂量氟尿嘧啶对肝癌细胞HepG2生物学行为的影响与^(18)F-FDG摄取关系的研究

Correlation between ^(18)F-FDG uptake and effect of small dose 5-Fu on biological behaviors of liver cancer HepG2 cells

目的研究小剂量氟尿嘧啶处理肝癌细胞HepG2后部分生物学行为与其摄取氟代脱氧葡萄糖(18F-FDG)变化的关系。方法使用低浓度的5-Fu(1μg/ml)培养肝癌细胞系HepG2,持续培养4周期后(第4周期后细胞简称HepG2/4P),观察细胞形态,绘制生长曲线,计算倍增时间;用Western blot检测Ki67的表达;于体外进行18F-FDG细胞放射性核素摄取实验。结果HepG2与HepG2/4P组的倍增时间分别为16.2h、25.2h;Ki67相对表达量分别为0.367±0.005、0.270±0.019,两组差异有统计学意义(P<0.05);HepG2/4P对18F-FDG摄取率较化疗前HepG2摄取降低,但无统计学意义(P=0.093)。结论HepG2/4P细胞与HepG2相比倍增时间延长,Ki67的表达降低,对18F-FDG摄取降低,提示肝癌细胞增殖的差异可能会引起肿瘤糖代谢相应的差异,可能用于监测化疗疗效和预测细胞生物学行为的改变。

Objective To study the correlation between 18F-FDG uptake and effect of small dose 5-Fu on biological behaviors of liver cancer HepG2 cells.Methods Morphological changes of liver cancer HepG2 cells were observed 4 weeks after they were cultured with 1μg/ml 5-Fu（expressed as HepG2/4P）.Then,their growth curve was plotted to calculate the doubling time of their growth.Expression of Ki67 was detected by Western blot.Uptake of 18F-FDG in vitro was detected using radioactive nuclide.Results The doubling time of HepG2 and HepG2/4P was 16.2h and 25.2h,respectively.The expression of Ki67 was 0.367±0.005 and 0.270±0.019,respectively（P0.05）.The uptake of 18F-FDG was lower by HepG2/4P than by HepG2（P=0.093）.Conclusion The doubling time of HepG2/4P is longer than HepG2 while the expression level of HepG2/4P is lower than that of HepG2 and the uptake of 18F-FDG is lower by HepG2/4P than by HepG2,suggesting that differences in proliferation of liver cancer cells can lead to corresponding differences in glucose metabolism,and uptake of 18F-FDG can be used as an indicator in monitoring chemotherapy and biological behaviors of liver cancer cells.