Bax基因在人颈动脉粥样硬化斑块中的表达

Bax gene expression in human carotid atheromatous plaques

目的研究Bax基因在稳定和不稳定粥样斑块中的表达及临床意义。方法颈动脉内膜剥脱术标本25例,由病理学分成稳定性(14例)和不稳定性(11例)两组,对照组正常动脉取自肝移植供者的腹主动脉及其分支(6例);进行免疫组化,原位杂交和原位凋亡DNA缺口末端标记法(TUNEL)测定Bax凋亡基因表达。结果稳定性斑块Bax阳性表达免疫组化5例,原位杂交5例,TUNEL阳性4例;不稳定性斑块Bax阳性表达免疫组化10例,原位杂交11例,TUNEL阳性表达9例,表达显著高于稳定性斑块(P<0.01);免疫组化和原位杂交阳性细胞半定量计数在稳定性斑块中分别为8.63±2.62和10.32±3.12,在不稳定性斑块分别为122±21.64和152±23.35,高于稳定性斑块(P<0.01);对照组无表达。结论Bax高表达是影响粥样斑块稳定性的分子病理学基础和脑卒中发生率的因素之一,在不同时期控制其表达可为基因治疗颈动脉粥样硬化提供靶点。

Objective To study the Bax expression in stable and unstable carotid atheromatous plaques and its clinical significance.Methods Twenty-five carotid atheromatous plaque specimens were divided into stable group（n=14）and unstable group（n=11）.Liver transplantation donors served as a control group（n=6）.Expression of apoptotic Bax was detected by immunohistochemistry（IHC）,in situ hybridization（ISH）and in situ TdT dUTP nick end labeling（TUNEL）,respectively.Results Positive expression of Bax in stable carotid atheromatous plaques was detected in 5 cases by ICH,in 5 cases by ISH,and in 4 cases by TUNEL,respectively,while in unstable carotid plaques was detected in 10 cases by IHC,in 11 cases by ISH,and in 9 cases by TUNEL,respectively（P0.01）.The intensity of Bax（＋）cells detected by IHC and ISH was 8.63±2.62 and 10.32±3.12 in stable carotid atheromatous plaques,and 122±21.64 and 152±23.35 in unstable carotid atheromatous plaques,respectively（P0.01）.No expression of Bax was found in control group.Conclusion A high expression level of Bax in unstable carotid atheromatous plaques is one of the factors influencing the molecular pathology of stability of carotid atheromatous plaques and neurological clinic events.Control of its expression in different stage of carotid atherosclerosis may provide targets in gene therapy for carotid atherosclerosis.