小剂量紫杉醇对乏氧Hela细胞放射敏感性影响的研究

The study of Low dose of Paclitaxel on radiosensitivity of human cervical cancer cell line Hela under normal and hypoxic growth conditions

目的探讨紫杉醇对正常和乏氧状态下人宫颈癌细胞Hela的放射增敏作用。方法细胞乏氧模型、细胞放疗、采用四甲基偶氮唑蓝(MTT)快速比色法检测细胞增殖率;结果不同浓度的紫杉醇作用于乏氧和正常状态下的Hela细胞,均表现出增殖抑制作用,且呈浓度依赖性,乏氧状态下的Hela细胞增殖率明显大于正常状态下的细胞,两者相比差异有统计学意义(P<0.05),说明乏氧细胞可对对化疗药物表现出一定的抵抗作用。小剂量的紫杉醇(1×10-3mg/L)可增加正常和乏氧状态下的He-la细胞对放射线的敏感性,其增殖率分别为(19.67±5.06)%、(12.38±3.55)%,且两者相比差异无统计学意义(P>0.05)。结论乏氧细胞对化疗药物有一定的抵抗作用;紫杉醇对乏氧及正常状态下的Hela细胞均有抑制作用,且呈现浓度依赖性;小剂量的紫杉醇对乏氧状态下的Hela细胞具有放射增敏作用。

Objective To investigate the radiation sensitizing effect of low does of paclitaxel on human cervical cancer cell line Hela under normal and hypoxia growth conditions.Methods The hypoxia model of Hela、Cell radiotherapy、 MTT assay was used to analyze cell proliferation rate.Results Different concentrations of PTX could inhibit the proliferation of Hela cells under different growth conditions,and it has dose-dependent effect.In the state of hypoxia,Hela proliferation was significantly higher than the normal state（P0.05）.Low does of PTX（1×10-3 mg/L） had a positive effect on the radiosensitivity of Hela under both normal and hypoxia growth conditions,the growth rate were （19.67±5.06）% and（12.38±3.55）% respectively（P0.05）;Conclusion Hypoxic cells have a certain resistance to chemotherapeutic drugs.PTX could inhibit the proliferation of Hela cells under different growth conditions,and it has dose-dependent effect.Low does of PTX could be used as a radiation sensitizer for Hela under both growth conditions.