神经生长因子对新生大鼠缺氧缺血性脑病的影响

Influence of Nerve Growth Factor on Hypoxic ischemic Encephalopathy of Newborn Rat

目的 探讨神经生长因子（ＮＧＦ）对新生大鼠缺氧缺血性脑病（ＨＩＥ）的影响。 方法 选用７ 日龄Ｗｉｓｔａｒ 大鼠，在制成ＨＩＥ模型前后给予腹腔注射ＮＧＦ各一次，在缺氧缺血后按不同时间分期处死，测定脑组织兴奋性氨基酸（ＥＡＡｓ） 及一氧化氮（ＮＯ） 的含量和凋亡细胞数，并与丹参组、生理盐水组的测定值比较。 结果 ＨＩＥ组ＥＡＡｓ、ＮＯ含量及凋亡细胞数明显高于正常对照组的测定值；ＮＧＦ干预组的测定值明显低于ＨＩＥ组及生理盐水组的测定值，而与丹参组的测定值相比无明显差别。 结论 ＮＧＦ 具有和丹参类似的作用，能阻断ＥＡＡｓ、ＮＯ 的异常代谢，降低细胞凋亡数，对ＨＩＥ具有防治作用。

Objective To investigate the prevention and treatment effect of nerve growth factor(NGF) on hypoxic ischemic encephalopathy. Methods NGF was injected intraperitoneally into 7 day newborn Wistar rats before and after hypoxia and ischemia.Rats were killed after HIE according to the different schedules.The contents of EAAs and NO in the left and right brains and the number of cell apoptosis were measured,which were compared to that of salivia militirrhixae group and normal saline(NS) group. Results The contents of EAAs,NO and the number of cell apoptosis were much higher in HIE group than those in normal control group.The values were much lower in NGF group than those in HIE group and NS group.There were no difference between NGF group and salivia militirrhixae group. Conclusion NGF was similiar to salivia militirrhixae on the affection of blocking abnormal metabolism of EAAs、NO and decreasing the number of cell apoptosis.NGF is of the prevention and treatment effect on hypoxic ischemic encephalopathy.