ICP-AES法研究不同配伍条件下白虎汤中钙离子溶出规律

Study on the Calcium Ion Dissolution Regularity of Different Compatibility in Baihu Decoction by ICP-AES

目的:通过研究不同配伍条件下药液中钙离子溶出量变化,探讨白虎汤的配伍规律。方法:采用电感耦合高频等离子体原子发射光谱(ICP-AES)法测定白虎汤中各单味药及不同配伍条件下药液中Ca2+溶出量。结果:各单味药物中均含钙,其中石膏含钙量最高,其次为知母和甘草,粳米含钙量最低。石膏与甘草配伍能增加药液中Ca2+溶出量,石膏与粳米配伍对药液中Ca2+溶出量影响不大,石膏与知母配伍可降低药液中Ca2+溶出量,全方配伍条件下药液中Ca2+溶出量增加。结论:这充分的体现了中医方剂组方原则,石膏为君药、知母为臣药发挥解热作用,甘草为佐药,抑制了部分知母对石膏中Ca2+溶出的负面作用,加强了君臣药的解热作用。研究结果显示,中药配伍有其规律性和合理性,这对进一步研究中药矿物药物在复方中药理作用有重要的指导意义。

Objective：To study the soluble calcium ion content of different compatibility of Baihu Decoction and to discuss the compatibility regularity of Baihu Decoction. Method：ICP-AES was used to detect the content of soluble calcium ion of each single and different compatibility herbs in Baihu Decoction. Result：The results showed that all of herbs in Baihu Decoction contained calcium. Gypsum had the highest content of calcium,followed by Rhizoma Anemarrhenae and Licoroce,Rice had the lowest. The calcium ion dissolution content was increased when Gypsum and Licoroce compatibled together; but not influenced by Gypsum and Rice and reduced by Gypsum and Rhizoma Anemarrhenae. Also the calcium ion content was increased when all herbs of Baihu Decoction compatibled together. Conclusion：It reflected the prescription principles of traditional Chinese medicine sufficiently. Gypsum is the monarch drug and Rhizoma Anemarrhenae is the minister drug,both of them have antipyretic effect. Licorice is assistant drug to restrain some Rhizoma Anemarrhenae＇s negative effects on the calcium ion solubility of gypsum,and increase the antipyretic effect of the monarch drug and minister drug. So the compatibility of traditional Chinese medicine has its own regularity and rationality,it has important guiding significance to the further research on the pharmacological effects of mineral drugs in Chinese herbal compound.