补阳还五汤促进大鼠腓总神经再生的实验研究

The Experimental Study on Regeneration of Rat Common Peroneal Nerve Promoted by Buyang Huanwu Decoction

目的:研究补阳还五汤(BYHWD)促进大鼠夹伤腓总神经再生的作用。方法:雄性SPF级SD大鼠60只,随机分为6组:BYHWD高、中、低剂量组(剂量分别为25.92,12.96,6.48g生药/kg)、弥可保组(剂量为625μg/kg)、模型组、假手术组。采用大鼠腓总神经5mm夹伤模型后,每日ig给药。术后18d行电生理检测,测定复合肌动作电位;组织形态学分析,测定再生有髓神经纤维数、髓鞘厚度,观察BYHWD对大鼠夹伤腓总神经的再生作用。结果:与模型组比,BYHWD高、中剂量组复合肌动作电位波幅、再生有髓神经纤维计数、髓鞘厚度均显著增高,有显著性差异。结论:BYHWD有利于轴突生长和髓鞘形成,可以促进大鼠夹伤神经再生。

Objective： To study the repairing effects of Buyang Huanwu Decoction （ BYHWD） on rat common peroneal nerve after peripheral nerve injury. Method： Common peroneal nerve injury induced by clamp was established for sixty Sprague-Dawley rats,then they were randomly divided into 6 groups for daily intragastric administration of drugs： BYHWD high-dose （25. 92 g /kg）,BYHWD medium-dose （12. 96 g /kg）,BYHWD lowdose （6. 48 g /kg）,mecobalamin （625 μg /kg） as the positive control,normal saline for model group and normal saline for the sham operation group. The drug administration lasted for 18 days. Electrophysiology was performed 18 days after operation to determine the compound muscle action potentials （ CMAPs）. The number of regenerated myelinated nerve fibers,thickness of myelin sheath and cross sectional area of tibial muscle were evaluated by histomorphology. Result： Amplitude of CMAP,the number of regenerated myelinated nerve fibers and thickness of myelin sheath in BYHWD high-dose and medium-dose group were all increased significantly compared with the model group. Conclusion： BYHWD contributes to axon growth and myelination reformation, thus promotes peripheral nerve regeneration in rats.