Aβ25-35刺激对海马神经细胞株NG108-15Toll样受体3、4表达的影响

Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells

目的:研究Aβ刺激对小鼠神经细胞株NG108-15Toll样受体(TLR)3、TLR4mRNA表达的影响。方法:Aβ刺激NG108-15细胞,24h后,取细胞培养上清液,ELISA法测定炎症因子TNF-α、IFN-β含量,荧光定量PCR法测定TLR3、TLR4mRNA表达量的变化。结果:Aβ诱导NG108-15细胞分泌TNF-α、IFN-β的量较正常对照组明显增多(均P<0.01);Aβ可诱导NG108-15细胞TLR3、TLR4mRNA表达增强,与空白对照组相比,均P<0.01。结论:Aβ刺激可体外诱导NG108-15细胞TNF-α、IFN-β分泌增多,诱导TLR3、TLR4mRNA表达增强,提示Aβ致阿尔茨海默病(AD)作用可能与免疫性炎症及TLRs信号转导通路有关。

Objective：To investigate the influences of Aβ25-35 on the TLR3 and TLR4 mRNA. Method： NG108-15 cell line was stimulated with Aβ25-35,the supernatant was collected 24 hours later and the inflammatory factors TNF-α and IFN-β were quantitatively measured,the changes in the mRNA expression of TLR3 and TLR4 were detected by real-time PCR. Result：Aβ induced the over-secreting of TNF-α、IFN-β and over-expression of TLR3 and TLR4 mRNA （P 0. 01）. Conclusions：Aβ induced the over-secreting of inflammatory factors and over-expression of TLR3 and TLR4,indicating the action of Aβ in causing AD disease might be affiliated with the immune inflammation and the TLRs signaling pathway.