新生儿溶血病检测试验多样性与胆红素水平相关性研究

Research on the relevance between different results of hemolysis detection tests and serum bilirubin levels of hemolytic disease of the newborn

目的探讨新生儿ABO溶血病（ABO．HDN）溶血三项试验不同结果与胆红素水平的相关性。方法对85例ABO—HDN患儿进行血清溶血三项试验[抗人球蛋白试验（DAT）、游离抗体试验、抗体释放试验]、母亲IgG抗A（B）抗体、血清胆红素等测定。结果ABO．HDN患儿DAT阳性者血清总胆红素峰值与DAT阴性者比较，差异有统计学意义（P〈0．05，0．01）；DAT阴性间比较、DAT阳性问比较（P〉0．05），差异无统计学意义。新生儿ABO．HDN患儿母亲血型抗体与血清总胆红素水平比较，差异无统计学意义（P〉0．05）。结论ABO-HDN患儿DAT阳性合并抗体释放试验阳性或三项试验均阳性时，血清胆红素水平增高较明显，DAT阴性释放试验和／或游离试验阳性时，新生儿ABO-HDN相对较轻，而母亲IgG抗体效价高低不能反映HDN的严重程度，这可为临床合理治疗HDN提供帮助。

Objective To study the relevance between different results of three hemolysis detection tests and serum bilirubin levels of ABO hemolytic disease of the newborn （ABO-HDN）. Methods Serological hemolysis three tests （direct anti-human globulin test, free antibody test, antibody release test）, maternal anti-A （B） IgG antibodies, serum bilirubin were examined in 85 cases of ABO-HDN. Results There were significant differences in levels of total serum bilirubin（TSB） between groups of DAT-positive and groups of DAT-negative （P 〈0.05 ,or P 〈 0. 01 ）. There weren＇ t signifieants difference between groups of DAT-positive or groups of DAT-negative in levels of total serum bilirubin （P 〉 0. 05 ）. There wasn＇ t significant difference between maternal antibodies titer and levels of total serum bilirubin （ P 〉 0. 05 ）. Conclusion When DAT is positive, the serum bilirubin level was significantly increased, and maternal antibodies titer eouldn＇ t show severity of ABO-HDN. It could be helpfull for the clinical treatment of ABO-HDN.