供体骨髓来源的未成熟树突状细胞抑制小鼠同种异体牙移植后排斥反应的效果研究

Suppression effects of immature dendritic cells on allogenic teeth rejection in mice

目的:研究供体小鼠骨髓来源的未成熟树突状细胞(Immatureden dritic cell,imDC)对小鼠同种异体牙移植后排斥反应的抑制效果。方法:运用50U/ml粒细胞-巨噬细胞集落刺激因子(Granulocyte-macrophage colony-stimulating factor,GM-CSF)诱导出小鼠骨髓来源imDC,流式细胞术鉴定细胞表型,并于同种异体牙移植前1周以2×106个/只回输入受者体内,组织病理切片观察imDC对小鼠同种异体牙移植后排斥反应的抑制效果。结果:imDC回输组与未回输imDC的同种异体牙移植组相比,淋巴细胞浸润减少、炎症反应减轻。结论:imDC可以减轻同种异体牙移植后的排斥反应。

Objective： To investigate the suppressive effects of immature dendritic cells derived from donor bone on mice allogenic teeth rejection. Methods： immature dendritic cells derived from mice bone marrow were generated with 50 U/ml granulocyte-macrophage colony-stimulating factor; the phenotype were analyzed by flow cytometry. 2×106 imDC per mouse were administered to the recipients through tail vein 7 days before allogenic teeth transplantation. The suppressive effects of imDC on transplantation rejection were observed through histological section. Results： The infiltration of lymph cell and Inflammatory reaction in allogenic teeth transplantation group were reduced after administration of imDC to the recipients.Conclusion：The allogenic teeth rejection could be lessened by imDC.