钙磷多孔陶瓷表面明胶处理及体外细胞相容性

Gelatin treatment of calcium phosphate porous ceramics surface and in vitro cytocompatibility

背景:烧结的钙磷多孔陶瓷由于其表面结构致密,在诱导细胞黏附和生长方面仍存在不足。目的:观察表面明胶处理对钙磷多孔陶瓷细胞相容性的影响。方法:以羟基磷灰石和磷酸钙为主要原料,采用有机泡沫浸渍工艺制备钙磷多孔陶瓷,然后将多孔陶瓷浸于5%的明胶溶液中进行表面处理。扫描电镜观察处理前后样品的孔隙形貌和表面结构,阿基米德法测试样品的孔隙率,WD-10A型电子万能材料试验机测定压缩强度;将材料与兔骨髓基质干细胞进行体外复合培养,通过MTT实验和扫描电镜观察细胞在材料上的生长情况。结果与结论:表面明胶处理后,多孔陶瓷的孔壁表面形成了较均匀的明胶涂层,样品的孔隙特征并没有受到显著影响。然而它们的平均压缩强度却从(1.04±0.15)MPa提高到了(5.17±0.17)MPa。扫描电镜观察和MTT实验结果表明,表面涂覆处理前后的多孔材料都具有良好的细胞相容性。与烧结的多孔陶瓷相比,表面明胶处理的样品更能增进细胞在材料表面的早期黏附和增殖。结果表明表面明胶处理在不破坏多孔陶瓷孔隙特征的情况下,不仅提高了钙磷多孔陶瓷的力学性能,而且改善了多孔陶瓷的细胞相容性。

BACKGROUND：Sintered porous calcium phosphate ceramics is still insufficient in the induction of cell adhesion and growth due to its dense surface structure.OBJECTIVE：To fabricate calcium phosphate porous ceramics with high porosity,to treat their surface by gelatin,and to study the influence of surface treatment on cytocompatibility.METHODS：Calcium phosphate porous ceramics were fabricated by the foam impregnation technology firstly,using HA and β-TCP as raw materials.Then the porous ceramics were treated by 5wt%gelatin solution.The changes of pore property and surface morphology were characterized by scanning electron microscopy（SEM）.The porosity of samples was measured by the Archimedes method.The compressive strength was measured with a WD-10A electronic universal material testing instrument.The rabbit bone narrow stromal cells were cultured on samples before and after surface treatment in vitro.MTT assay was performed for cells proliferation and SEM was used to observe the morphology of cells on porous ceramic.RESULTS AND CONCLUSION：After surface treatment,gelatin coatings formed uniformly on the pore walls of porous ceramic samples without changing their porous characteristics obviously.However,the average compressive strength value of samples was improved from（1.04±0.15）MPa to（5.17±0.17）MPa.In vitro,good cytocompatibility of samples before and after surface treatment was verified by the results of SEM and MTT.Moreover,the attachment,proliferation and activity of cells on the surface of samples treating with gelatin were better than those of sintered porous ceramics.Without destroying porous characteristic of samples,surface treatment with gelatin not only strengthened porous ceramics,but also improved cytocompatibility of the samples.