兔VX2肝癌动物模型的建立

Establishment of a VX2 Hepatic Cancer Model in Rabbits

目的:介绍应用VX2肿瘤组织块制作兔移植性肝癌模型方法。方法:新西兰大白兔26只,采用开腹瘤块包埋法接种兔VX2瘤于肝左叶。于移植后2-3周进行CT扫描及数字减影血管造影(diagnostic digital subtraction angiography,DSA),实验完成后处死实验兔进行病理学检查。结果:26只兔均接种成功,接种成功率100%。VX2肝癌在CT平扫呈低密度,在肝动脉期表现为不同程度的环形强化,在门脉期呈低密度,病灶与周围肝组织分界清楚。肝动脉数字减影血管造影可见肿瘤染色。结论:兔VX2肝癌的表现与人类原发性肝癌类似,是肝癌介入治疗实验研究的理想动物模型。

Objective： To introduce the establishment of a VX2 hepatic cancer model in rabbits.Methods： Twenty-six New Zealand white rabbits were implanted with VX2 tumor in the left lobe of the liver.CT scanning and digital subtraction angiography（DSA） were performed in all the rabbits 2 to 3 weeks after implantation.Results： All the twenty-six（100%） rabbits were successfully implanted with VX2 tumor in the liver.The tumors were demonstrated as hypodensity on plain CT images,ringe-enhanced on arterial phase images and hypodensity on portal phase images.Digital subtraction angiography of the hepatic artery confirmed the hypervascular mass with tumor vasculature.Conclusion： The VX2 hepatic tumors in rabbits demonstrate their hemodynamics as human primary hepatic carcinomas,and it is more suitable for interventional experimental study.