白细胞介素-18基因单核苷酸多态性及其血清含量与溃疡性结肠炎的相关性

Association of the single nucleotide polymorphism and the serum contents of interleukine-18 with ulcerative colitis

目的探讨白细胞介素（IL）-18基因单核苷酸多态性（SNP）及其血清含量与溃疡性结肠炎（UC）的相关性。方法采用PCR扩增和直接测序法检测50例uC患者及128名健康对照者的IL-18基因5’端4个SNP位点（rs187238G／C、rs5744228G／A、rs360718A／C、rs360717G／A），同时检测受试者血清IL-18含量。结果UC组血清IL18含量明显高于对照组，两组间差异有统计学意义（3029．9±111．7）pg／ml比（133．2±39．4）pg／ml，（P〈0．01）。rs187238C、rs360718C、rs360717A位点与UC显著相关（x2=9．26，P〈0．01），该3个位点的单倍体型频率在UC组和对照组间差异亦有统计学意义（x2=7．04，P〈0．01）。与对照组相比，UC组含等位基因型C者的血清IL-18含量明显升高（P〈0．05）。结论IL-18基因的SNP与其血清含量存在相关性，提示其基因位点多态性及血清含量可能与UC易感性相关。

Objective To estimate the association of the single nucleotide polymorphism （SNP） and the serum concentration of interleukin （IL）-18 with ulcerative colitis （UC） . Methods Samples collected from 50 patients with UC and 128 healthy controls were detected for SNPs of IL-18 gene including rs187238, rs5744228, rs360718 and rs360717 using polymerase chain reaction （PCR） and direct sequencing . Meanwhile, the serum level of IL-18 was measured. Results Serum concentration of IL-18 was higher in patients with UC than in healthy controls [（3029.9± 111.7） pg/ml vs （133.2± 39.4） pg/ml, （P〈0.01）]. rs187238 C, rs360718 C and rs360717 A were significantly related to UC （x2 =9.26, P〈0.01）. There was significant difference in frequencies of rs187238 C, rs360718 C and rs360717 A between patients with UC and healthy controls （X2 =9. 26, P〈 0. 01 ）. Serum concentration of IL-18 was higher in UC patients with C allele than in healthy controls （P〈0.05）. Conclusions There is correlation between SNP and serum level of IL-18 , which may involve in susceptibility to UC.