摘要
对接种和未接种B16黑色素瘤细胞的C57小鼠进行血清O-糖链比较糖组学研究,寻找黑色素瘤血清特异性O-糖链.小鼠血清10μL,β-消除反应释放O-糖链.反应混合物经石墨化炭黑固相萃取小柱(GCC SPE)分离纯化后,用于MALDI-Qit-TOF-MS分析.通过Launchpad软件采集并输出质谱数据,MATLAB进行数据解析,找到了10个稳定出现的差异糖链质谱峰.利用串联质谱分析了其中5个主要差异糖链的结构.
Glycomics comparison was carried out for screening melanoma serum biomarkers between C57 mouse with and without B16 implanted.O-glycans were released from 10 μL serum by β-elimination,purified by GCC SPE and analyzed by MALDI-QIT-TOF-MS.MS raw data was acquired and exported by Launchpad software.MATLAB was then applied for further data analysis.10 glycans were considered to be firmly changed after B16 implantation and 5 of them were picked for MS/MS for structure analysis.
出处
《中国科学:化学》
CAS
CSCD
北大核心
2010年第5期517-522,共6页
SCIENTIA SINICA Chimica
基金
上海市教育委员会科研创新项目(10YZ212)
国家自然科学基金项目(30900254)资助