INK4系列抑癌基因纯合子缺失、甲基化与白血病预后的实验研究

Study on pl6, p15, p18 and p19 homozygous deletion and methylation in leukemia

目的研究INK4系列抑癌基因纯合子缺失、甲基化与白血病预后的关系。方法采用聚合酶链反应（PCR）研究p16基因家族在白血病中纯合子缺失，应用甲基化敏感限制内切酶HpaII结合PCR技术研究自血病患者p16、p15、p18、p19基因甲基化状况，用单因素、多因素Logistic回归分析其基因失活与急性白血病（AL）预后的关系。结果基因表达组治疗有效27例（84．38％），基因失活组治疗有效11例（28．95％），基因表达组治疗有效率明显高于基因失活组（P〈0．001）。单因素、多因素Logistic回归分析结果显示p16、p15基因失活化疗有效率明显低于基因表达组。结论p16、p15基因失活可作为AL病程进展、复发、预后的指标之一。

Objective To explore the correlation between the family of pl6 gene inactivation and prognosis of leukemia, and then to clarify, the pathogenesis of leukemia, and monitor process of leukemia. Methods We used polymerase chain reaction（PCR） to study p16, p15, p18, p19 gene homozygous deletion, by using methylation-sensitive enzyme and PCR technology to iuvestigate p16, p15, p18, p19 gene methylation in leukemia. Results The effective rate with p16 and p15 gene aetiviation was 27 cases （84.38 %）, the effective rate with p16 and p15 gene inaetiviation was 11 cases （28.95 %）, and the total effective rate with p16 and p15 gene aetiviation was higher than p16 and p15 gene inactivation.In case to use single and muhi factor Logistic regression, effective rate in cases with p16 and p15 gene inactiviation was lower than that with p16 and p15 gene activation. Conclusion It might be one of parameters for forcasting progression, relapse and prognosis in AL.