摘要
为使产油真菌浅白隐球酵母的油脂更适合生物柴油的要求,利用根癌农杆菌介导的遗传转化方法,将植物SAD基因转入浅白隐球酵母中,并对影响转化效率的关键因子进行了优化。结果表明:利用农杆菌菌株AGL-1介导,农杆菌浓度为1×109个/mL,酵母浓度为酵母密度为1×107～2×107个/mL,在含有AS200μmol/L条件下共培养48h可获得较高的转化率。PCR检测表明,SAD基因已成功转入浅白隐球酵母中,证明了该转化系统对浅白隐球酵母遗传转化的可行性。
Agrobacterium tumefaciens-mediated transformation method was used to transform plants SAD gene into Cryptococcus albidus.Key factors affecting transformation were optimized.The results showed that when cultured sample for 48 h under the conditions that mediated by Agrobacterium tumefaciens strain（AGL-1）,concentration of Agrobacterium tumefaciens was 1×109 strains/mL,concentration of Cryptococcus albidus was 1×107～2×107 strains/mL and concentration of AS was 200 μmol/L,the transformation rate was higher.PCR detection determined that SAD gene has been transformed into Cryptococcus albidus.All the results above proved that the ATMT system is feasible to genetic transformation of Cryptococcus albidus.
出处
《湖南农业科学》
2010年第4期16-19,共4页
Hunan Agricultural Sciences
基金
浙江省重大科技攻关项目(2006C120301)
关键词
根癌农杆菌
SAD
遗传转化
浅白隐球酵母
Agrobacterium tumefaciens
SAD
genetic transformation
Cryptococcus albidus
