慢性疼痛应激对雄性SD大鼠生殖相关参数的影响

Effect of chronic pain stress on reproductive related parameters

目的观察不同时间段慢性疼痛应激后,SD大鼠生殖相关参数的变化。方法建立坐骨神经分支选择性损伤模型。经不同时间疼痛应激后,于14 d、21 d和28 d处死大鼠,取睾丸、附睾称重,并对附睾尾精子计数,HE染色显示睾丸结构变化,western blot检测3 β-HSD蛋白表达的改变。结果 14 d和21 d手术组大鼠体重均略低于对照组,但差异无统计学意义(P>0.05)。不同时间段各组大鼠睾丸和附睾系数均无明显变化。28 d后手术组附睾尾精子相对计数显著低于对照组(P<0.05)。HE染色显示,坐骨神经分支选择性损伤诱导疼痛应激28 d后睾丸生精上皮中生精细胞减少,生精小管腔内精子数量减少。Western blot结果显示28 d手术组3 β-HSD表达显著低于正常照组和假手术组(P<0.05)。结论慢性疼痛应激如其他方式应激一样,能引起大鼠附睾尾精子相对计数减少,睾丸生精上皮中生精细胞减少,睾丸内3 β-HSD含量降低,慢性疼痛应激降低了大鼠的生精功能。

Objective To study the changes of reproductive related parameters during different term chronic pain stress, including the weight of testis and epididymis, sperm amount in cauda epididymidis and the expression of 3 beta-hydroysteroid dehydrogenase （3β-HSD） in testis. Methods The established rat model with Spare nerve injury was stimulated with different chronic pain stress. On 14th day, 21 th day and 28th day, the rats were sacrificed respectively. The testis and epididymis were isolated and weighted, and the number of epididymal sperm was calculated. The structure of testis was analyzed by HE stains. The expresssion of 3 beta-hydroysteroid dehydrogenase expression in testis was detected by Western blot. Results （1） The body weights on day 14 and 21 were decreased in stress group compared with that in normal and sham group. However no statistical significance in body weights was found among these groups. No obvious changes in testicular and epididymal coefficients among these groups were found during different time points. （3） The number of spermatogenic cell in seminiferous epithelium and number of sperm in seminiferous lumens was all decreased. （4） the expression of 3β -HSD on 28 d in stress group was significantly lower than that in normal and sham group. Conclusion Chronic pain stress might reduce the weight of testis and epididymis, the unmber of sperm in cauda epididymidis and the expression of 3β-HSD in testis. In conclusion, chronic pain stress might weaken the reproduction function of rat.