不同碱性药物对急性肺损伤小鼠病死率的影响

Effects of different alkali on the survival of acute lung injury mice

目的:探讨碳酸氢钠和三羟基氨基甲烷(THAM)对急性肺损伤小鼠病死率的影响。方法:采用脂多糖致小鼠急性肺损伤模型,144只小鼠随机分为空白对照组、肺损伤组、碳酸氢钠组和THAM组,各组经尾静脉泵注不同药物后随机抽取6只小鼠,行血气分析及肺组织学检查。其余120只小鼠观察72h病死率。结果:与空白对照组相比,肺损伤组pH和PO2明显下降(P<0.05);碳酸氢钠组、THAM组与肺损伤组相比pH和PO2明显升高(P<0.05)。与肺损伤组相比,THAM组72h病死率明显降低(P<0.05)。肺损伤组与碳酸氢钠组、THAM组肺内白细胞浸润和肺水肿程度无明显差异。结论:THAM治疗急性肺损伤小鼠可提高其生存率。

Objective To investigate the effects of sodium bicarbonate （NaHCO3） and THAM on the mortality rate in mice with acute lung injury. Methods The murine model of acute lung injury （ALI） was established by using LPS. 144 mice were randomized to control group, ALI group, THAM group, or NaHCO3 group. After treatment, blood gas analysis and histological examination of lung were performed in Six mice in each group. The mortality rate was observed in the remaining 120 mice for 72 hours. Results As compared with the control group, PH value and PO2 were markedly decreased in ALI group （P 〈 0.05）. PH value and PO2 were obviously elevated in both THAM and NaHCO3 group as compared with ALI group （P 〈 0.05）. The mortality rate was significantly lower in THAM group than in ALI group （P 〈 0.05）. There were no significant differences in intrapulmonary leukocyte infiltration and pulmonary edema between NaHCO3 and ALl group. Conclusions THAM therapy can increase the survival rate in mice with acute lung injury.