天南星药材的鉴别及黄酮含量测定方法研究

Study on Identification of Rhizoma Arisaematis and Content Determination of Flavonoids

目的:建立天南星药材的鉴别方法及黄酮含量测定方法。方法:以夏佛托苷和异夏佛托苷为标准品,采用薄层色谱(TLC)法鉴别天南星药材;采用高效液相色谱法测定天南星中黄酮的含量。结果:TLC中,供试品在与标准品色谱相应的位置上,显相同颜色的斑点。夏佛托苷和异夏佛托苷分别在7.925～126.8μg·mL-(1r=0.9999)、3.996～63.94μg·mL-(1r=0.9997)浓度范围与各自峰面积积分值之间呈良好的线性关系;平均回收率分别为99.40%(RSD=2.10%,n=9)、99.52%(RSD=2.42%,n=9)。结论:本方法操作简便、准确性高、重复性好,可作为天南星药材质量评价的方法。

OBJECTIVE：To establish the identification method of Rhizoma Arisaematis and content determination of flavon-oids.METHODS：TLC was used to identify Rhizoma Arisaematis with schaftoside and ischaftoside as reference substances.The content of flavonoids was determined by HPLC.RESULTS：TLC of test sample and that of control substance had same color dots.The linear range of schaftoside and isoschaftoside were 7.925～126.8 μg·mL^-1（r=0.999 9） and 3.996～63.94 μg·mL^-1（r=0.999 7） respectively.Average recoveries were 99.4% for schaftoside （RSD=2.10%,n=9） and 99.52% for isoschaftoside（RSD=2.42%,n=9）.CONCLUSION：The method is simple,accurate and reproducible for the quality evaluation of Rhizoma Arisaematis.