摘要
目的:测定何首乌中9种有机氯农药残留量。方法:样品用乙酸乙酯超声提取并经Florisil固相萃取柱净化后,采用毛细管气-质联用仪测定有机氯农药残留量:色谱柱为DB-5MS弹性石英毛细管柱(30m×0.25mm,0.25μm),进样口温度为230℃,程序升温(初始温度100℃,以8℃·min-1升温至220℃,再以5℃·min-1升温至250℃,保持10min),载气流速为1mL·min-1,进样量为1μL。结果:9种有机氯农药能在30min内完全分离;平均回收率为80.4%~97.2%,RSD为3.5%~7.4%(n=6)。结论:本方法灵敏、准确,符合多种有机氯农药残留的检测要求。
OBJECTIVE:To determine the residues of 9 kinds of organochlorinated pesticides in Polygonum multiflorum.ME-THODS:Samples were extracted with ethyl acetate by ultrasonic wave assistant extraction and purified on Florisil solid-phase extraction column.The residues of organochlorinated pesticides were determined with capillary gas chromatography with GC-MS.The separation was performed on DB-5MS(30 m×0.25 mm,0.25 μm) fused-silica capillary column with injector temperature of 230 ℃.The initial column temperature was 100 ℃,then raised to 220 ℃ at the rate of 8 ℃·min^-1.The column temperature was ra-ised at 250 ℃ at the rate of 5 ℃·min^-1 for 10 min.The column flow was 1 mL·min^-1 and injection volume was 1 μL.RESULTS:9 kinds of organochlorinated pesticides were completely separated within 30 minutes.The average recoveries ranged from 80.4% to 97.2%(RSD ranged from 3.5% to 7.4%,n=6).CONCLUSION:The method is sensitive and accurate for the content determination of the residues organochlorinated pesticides.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第19期1777-1778,共2页
China Pharmacy
基金
广东省科技计划项目(2007B031407003)
广州市科技计划项目(2007Z2-E5061)
关键词
何首乌
有机氯农药
残留
固相萃取
气-质联用法
Polygonum multiflorum
Organochlorinated pesticides
Residues
Solid-phase extraction
GC-MS