萼花臂尾轮虫(Brachionus calyci florus)的ISSR引物筛选及其多态性检验

PRIMER SCREENING AND POLYMORPHISM DETECTION OF ISSR MARKERS IN THE ROTIFER BRACHIONUS CALYCIFLORUS

采用简单重复序列区间(ISSR:一种基于微卫星的分子标记,无需预知遗传背景即可研究基因组中微卫星序列的变异,适合大规模的种群遗传研究)的方法对萼花臂尾轮虫(Brachionus calyci florus)进行PCR扩增,优化出适宜的ISSR-PCR反应体系,并从100条引物中筛选出16条具有良好多态性和重复性的ISSR引物.研究结果显示,萼花臂尾轮虫简单重复序列以(AC)和(AG)的两碱基重复为主,筛选出的ISSR引物扩增共得到114个位点,66个多态位点,多态性高达57.89%,高于常用于该物种分子生态学研究的线粒体COI基因的多态性.

Inter-simple sequence repeats（ISSR） is a novel molecular marker based on SSR,and is suited for large scale genetic surveys of plant and animal population.ISSR was used to detect genetic variation in rotifer Brachionus calyciflorus,and PCR reaction was optimized.Sixteen ISSR primer pairs screened from 100 generated a total of 114 bands,with 66 polymorphic bands.These data demonstrated that simple sequence repeats in rotifer B.calyciflorus were mainly composed of di-nucleotide repeats of（AC） and（AG）.