铜胁迫对国槐幼苗生长及叶片生理特性的影响

Effect of copper stress on the growth and physiological characteristics of leaves in Sophora japonica seedlings

采用盆栽方法研究了不同浓度处理水平的铜对国槐（Sophorajaponica）生长及叶片部分生理指标的影响。结果表明：国槐幼苗的株高及地径随铜胁迫浓度的增加表现出先增加后下降的趋势，在浓度1000mg·kg-1时，株高和地径与对照相比达到极显著差异（检验水平，P〈0．01）。在浓度〈500mg·kg-1时，叶绿素含量的呈下降趋势，细胞膜透性呈增加趋势，与对照相比差异不显著；脯氨酸含量与超氧化物歧化酶（SOD）活性迅速上升，与对照处理相比差异显著，表现出一定的适应能力。随着铜胁迫浓度的增加（〉500mg·kg-1），叶绿素含量及SOD活性显著下降，MDA含量、相对电导率、脯氨酸含量显著上升，表明国槐幼苗受害明显加重。

We studied the effect of copper stress on the growth and some leaves physiological indexes of Sophora japonica under different concentration treatments by using pot-culture method. The results showed that： the plant height and ground diameter of Sophora japonica exhibited an increasing trend followed by declining with increasing copper stress. When the concentration of copper were 1000 mg.kg-1, the plant height and ground diameter had an extremely significantly difference respectively in comparison with control. When the concentration of copper lower than 500 mg.kg-1, the chlorophyll contents increased rapidly, exhibiting an increasing tendency, and there were no significant difference in comparison with control. The proline contents and superoxide dismutase （SOD） activity increased rapidly, significantly different from the control, and exhibited some adaptability. With increasing concentrations of copper stress （ 〉500 mg.kg-1） , the contents of chlorophyll and activities of SOD decreased remarkably, and the MDA, relative conductivity and proline contents ascended sharply, indicating Sophorajaponica suffer significant injury.