投喂频次对凡纳滨对虾行为及免疫功能的影响

Effects of feeding frequencies on behaviors and immune function of Litopenaeus vannamei (Boone)

对凡纳滨对虾在每日不同投喂频次（1次／d、2次／d、6次／d），每次过量投饵条件下的行为、生长和免疫指标进行了研究。结果显示：随着投喂频次增加，凡纳滨对虾的活动水平和昼夜游走距离均呈逐渐升高的趋势，特定生长率显著升高。在白昼，6次·d。组对虾的活动频率显著高于其它组（p〈0．05）；在夜间，2次·d。组的活动频率显著高于其它组（p〈0．05）。1次·d。组对虾的酚氧化酶PO、过氧化氢酶CAT和超氧化物歧化酶SOD活力下降，丙二醛MDA含量升高：2次·d。组凡纳滨对虾的CAT活力、SOD活力和MDA含量在实验期间趋于稳定；6次·d。组免疫酶活力呈缓慢升高的态势。

The experiment was conducted for 30 days in order to determine the effects of different feeding frequencies （1, 2 and 6 times per day） on behaviors, growth and non-special immunity factors of Litopenaeus vannamei. The specific growth rate, movement time and movement distances increased progressively as feeding frequency increased. In daytime, the movement frequencies in the group fed for 6 times per day were significantly higher than those in other groups （p〈0.05） .However, at night, the movement frequencies in the group fed for 2 times per day were significantly higher than those in other groups （p〈0.05） . When feeding 1 time per day, the activities of phenoloxidase （PO）, catalase（CAT） and superoxide dismutase （SOD） of the shrimp declined, but malondialdehyde （MDA） level increased. During the experiment, the values of PO, CAT, SOD and MDA of the shrimps fed 2 times per day did not change obviously, but they grew slowly when fed 6 times per day.